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T. thermophilus HB8 alternative sigma factor sigE (TTHB211) deletion mutant strain, TT medium, 8 h

ABSTRACT: We observed the expression profile of the total mRNA of sigE (TTHB211) deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70 degC.

ORGANISM(S): Thermus thermophilus HB8

PROVIDER: GSE21436 | GEO | 2010/10/15

SECONDARY ACCESSION(S): PRJNA129479

REPOSITORIES: GEO

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T. thermophilus HB8 alternative sigma factor sigE (TTHB211) deletion mutant strain, TT medium, 8 h

Project description:We observed the expression profile of the total mRNA of sigE (TTHB211) deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70 degC. Three TTHB211 deletion mutant T. thermophilus HB8 strains were each pre-cultured at 70 degC for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70 degC. Cells were collected after 8 h, and then crude RNA was extracted. The expression of each mRNA were analyzed on a GeneChip.

2010-10-15 | E-GEOD-21436 | biostudies-arrayexpress

Comparative expression analysis between sigE (TTHB211) deletion mutant and wild-type of T. thermophilus HB8

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Comparative expression analysis between sigE (TTHB211) deletion mutant and wild-type of T. thermophilus HB8

Project description:We observed the expression profile of the total mRNA of sigE (TTHB211) deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70oC compared with that of wild type. Keywords: cell type comparison Three wild-type and mutant T. thermophilus HB8 strains were each pre-cultured at 70oC for 16 h in 3 ml of TT medium containing 0.8% polypeptone, 0.4% yeast extract, 0.2% NaCl, 0.4 mM CaCl2, and 0.4 mM MgCl2, which was adjusted to pH 7.2 with NaOH. The cells (2 ml) were inoculated into 1 liter of the same medium and then cultivated at 70oC. Cells were collected after 8 h, and then crude RNA was extracted. In order to compare mRNA expression in the sigE (TTHB211) deletion mutant with that of wild type, the expression of each mRNA at the time point was analyzed on a GeneChip as described under Sample Description Sheet of each sample in this website.

2007-09-04 | E-GEOD-8780 | biostudies-arrayexpress

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Project description:We observed the expression profile of the total mRNA of crp (TTHA1437) deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70 degC

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mRNA expression in wild-type of Thermus thermophilus HB8 strain grown on a rich (TT) medium at 45˚C and 70˚C

Project description:We observed the expression profile of the total mRNA of wild-type of Thermus thermophilus HB8 strain grown in a rich (TT) medium at 45˚C compared with that of 70˚C. Keywords: Growth temperature

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SuperSeries for the study of expression analysis of the PfmR (TTHB023) in T. thermophilus HB8

Project description:This SuperSeries is composed of the following subset Series: GSE21290: Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown in a rich medium (labeling:Affymetrix) GSE36881: Expression profile of PfmR (TTHB023)-deficient Thermus thermophilus HB8 strain GSE36883: Expression profile of wild-type Thermus thermophilus HB8 strain cultured in TT medium for 6 h, reproduced in 2010 GSE36884: Expression profile of wild-type Thermus thermophilus HB8 strain cultured in TT medium for 6 h, reproduced in 2011 Refer to individual Series

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SuperSeries for the study of expression analysis of the T. thermophilus sigE (TTHB211)

Project description:This SuperSeries is composed of the following subset Series: GSE7165: mRNA expression in wild-type Thermus thermophilus HB8 strain grown on a rich medium. GSE8780: mRNA expression in sigE (TTHB211) deletion mutant of Thermus thermophilus HB8 strain grown on a rich medium Keywords: SuperSeries Refer to individual Series

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Comparative expression analysis between TTHA0101-deficient mutant and wild-type of Thermus thermophilus HB8

Project description:We observed the expression profile of the total mRNA of TTHA0101 deletion mutant of Thermus thermophilus HB8 strain grown in a rich (TT) medium at 70°C compared with that of wild type.

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Comparative expression analysis between PfmR (TTHB023) deletion mutant and wild-type of Thermus thermophilus HB8

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Time course of the mRNA expression in wild-type Thermus thermophilus HB8 strain grown on a rich medium (labeling:ENZO)

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