Other

Dataset Information

0

Influence of Staphylococcus aureus strain background on Sa3 phage life cycle switches


ABSTRACT: Staphylococcus aureus (S. aureus) is a known pathogen able to infect humans and animals. Human S. aureus isolates are often associated with carriage of Sa3int prophages combined with loss of beta-hemolysin production due to gene disruption, whereas animal isolates are positive for beta-hemolysin associated with absence of Sa3int prophages. Sa3int prophages are known to contribute to staphylococcal fitness and virulence in human host by providing human-specific virulence factors encoded on the prophage genome. Strain-specific differences in regard to phage transfer, lysogenization and induction are attributable to yet unknown staphylococcal factors specifically influencing prophage gene expression. In this work we used tagRNA-sequencing approach to specifically search for these unknown host factors and differences in prophage gene expression. For this purpose, we established a workflow revealing the first direct comparison for differential gene expression analysis on two distinct single-lysogenic S. aureus isolates. Further, global gene expression patterns were investigated in two S. aureus isolates upon mitomycin C treatment and compared to uninduced conditions. This provides new insights into the tightly linked host-phage interaction network.

ORGANISM(S): Staphylococcus aureus

PROVIDER: GSE214523 | GEO | 2022/10/03

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2011-02-28 | E-GEOD-26623 | biostudies-arrayexpress
2011-12-31 | E-GEOD-26585 | biostudies-arrayexpress
2011-12-31 | GSE26585 | GEO
2011-12-31 | GSE26756 | GEO
2011-02-28 | GSE26623 | GEO
2023-02-03 | PXD036676 | Pride
2018-08-20 | GSE111012 | GEO
2011-04-15 | E-BUGS-115 | biostudies-arrayexpress
2015-04-01 | GSE60543 | GEO
2022-03-23 | GSE190637 | GEO