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Tri-modal Single Cell Approach Reveals Cellular and Molecular Heterogeneity in Naïve T cells Across Human Age

ABSTRACT: The naïve T cell compartment undergoes multiple changes across age that associate with altered susceptibility to infection and autoimmunity. In addition to the acquisition of naïve-like memory T cell subsets, recent murine studies describe substantial molecular reprogramming of the naïve compartment in adults compared with adolescents. However, these alterations are not well delineated in human aging. Using a new trimodal single cell technology (TEA-seq), we discovered that the composition as well as the transcriptional and epigenetic programming of the naïve T cell compartment in children (11-13 yrs) is distinct from that of older adults (55-65 yrs). Naive CD4 T cells, previously considered relatively resistant to aging, exhibited far more pronounced molecular reprogramming than the CD8 compartment, in which alterations are preferentially driven by shifts in naive-like memory subsets. These data reveal the complex nature of the naïve T cell compartment that may contribute to differential immune responses across the spectrum of human age.

ORGANISM(S): Homo sapiens

PROVIDER: GSE214546 | GEO | 2023/08/31

REPOSITORIES: GEO

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