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Dmrt1 regulates mouse expanded potency and facilitates the breaking of embryonic lineage barrier [RNA-Seq]

ABSTRACT: RNA-seq for EPSCM; Bulk RNA-seq were performed on E14 cells cultured in ES, 2iLIF and EPSCM

ORGANISM(S): Mus musculus

PROVIDER: GSE215141 | GEO | 2025/04/01

REPOSITORIES: GEO

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Dmrt1 regulates mouse expanded potency and facilitates the breaking of embryonic lineage barrier [ATAC-Seq]

Project description:ATAC-seq for E14 cells cultured in ES, 2iLIF and EPSCM;ATAC-seq was performed on cells E14 cells cultured in ES, 2iLIF and EPSCM

2025-04-01 | GSE215142 | GEO

Dmrt1 regulates mouse expanded potency and facilitates the breaking of embryonic lineage barrier [ChIP-Seq]

Project description:ChIP-seq for ES, 2iLIF, EPSC; Histone ChIP-seq was performed in E14 cells cultured in ES, 2iLIF and EPSCM

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Dmrt1 regulates mouse expanded potency and facilitates the breaking of embryonic lineage barrier

Project description:This SuperSeries is composed of the SubSeries listed below.

2025-03-31 | GSE215205 | GEO

Dmrt1 regulates mouse expanded potency and facilitates the breaking of embryonic lineage barrier

Project description:Dmrt1 regulates mouse expanded potency and facilitates the breaking of embryonic lineage barrier

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Dmrt1 regulates mouse expanded potency and facilitates the breaking of embryonic lineage barrier [ChIP-Seq]

Project description:Dmrt1 regulates mouse expanded potency and facilitates the breaking of embryonic lineage barrier [ChIP-Seq]

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Dmrt1 regulates mouse expanded potency and facilitates the breaking of embryonic lineage barrier [ATAC-Seq]

Project description:Dmrt1 regulates mouse expanded potency and facilitates the breaking of embryonic lineage barrier [ATAC-Seq]

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Dmrt1 regulates mouse expanded potency and facilitates the breaking of embryonic lineage barrier [RNA-Seq]

Project description:Dmrt1 regulates mouse expanded potency and facilitates the breaking of embryonic lineage barrier [RNA-Seq]

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Transcription Factor Co-Expression Mediates Lineage Priming for Embryonic and Extra-Embryonic Differentiation [scRNA-Seq]

Project description:In this study, we characterised transcriptomes of mESC in different conditions EPSCM and KOSR subsetting them for their single expression of SOX2 or the double expression of SOX2 and GATA6 in these conditions. 2iLIF (sorted only by DAPI negative cells) were used as control.

2023-12-05 | GSE227888 | GEO

Human pluripotent stem cell derived hematopoietic cells using expanded potential stem cell media

Project description:We recently developed a novel chemical media formulation, Expanded Potential Stem Cell Medium (EPSCM), that maintains mouse pluripotent stem cells (PSCs) in a unique cellular state that affords extraembryonic differentiation capacity. Here, we have used EPSCM to stably maintain human PSCs and used EPSCM-maintained iPSCs (EC-EPSCM) to study in vitro hematopoietic differentiation by embryoid body (EB) formation. We found that human PSCs maintained in EPSCM can spontaneously form embryoid bodies and efficiently undergo in vitro hematopoiesis using a simple differentiation protocol. EC-EPSCM PSCs generated primitive-wave and definitive-wave hematopoietic cells, which were chracterized by RNA-sequencing analysis.

2022-04-26 | GSE130662 | GEO

Breaking the epigenetic barrier during reversion of post-implantation epiblast cells

Project description:Primitive ectoderm cells (PE) in blastocysts represent the foundation of the pluripotent state, which is lost progressively during development. For example, development of epiblast cells from PE in postimplantation embryos is accompanied by transcriptional and epigenetic changes, including DNA methylation and X inactivation (Refs); these changes alter the nature of epiblast cells fundamentally, affecting their responsiveness to signaling molecules, and constitute a robust boundary that prevents their reversion to a PE-like state. Notably, epiblast cells unlike PE, are refractory to leukaemia inhibitory factor (LIF)/STAT3 signalling in vitro; instead, they respond to FGF/Activin to form self-renewing epiblast stem cells (EpiSCs) that are like human ES cells, which differ significantly from mouse embryonic stem cells (ES) derived from PE. However, here we show that under appropriate conditions, epiblast cells from postimplantation embryos can respond to LIF/STAT3/fetal calf serum (FCS), and undergo reprogramming to form embryonic stem cell -like cells (repiES: reprogrammed epiblast ES-like cells). Reprogramming of epiblast cells occurs progressively. First, they form colonies that retain key properties of epiblast cells (cEpi: cultured epiblast), which subsequently show erasure of epigenetic modifications, including DNA demethylation and X-reactivation to generate repiES. Analysis also revealed that repiES progressively acquire a transcriptome profile of ES cells that is distinct from cEpi and EpiSCs. In chimeras, repiES contributed to all the tissues, including germ cells. Thus, we show for the first time that reversion of epiblast cells to repiES phenotype entails progressive loss of phenotypic and epigenetic memory of epiblast cells. Our study provides insights into underlying mechanisms, and a tractable model for how signaling molecules induce epigenetic reprogramming of cells leading to an elemental pluripotent state.

2009-10-29 | GSE15487 | GEO

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