Transcriptomics

Dataset Information

0

Single-cell profiling identifies ACE+ granuloma macrophages as a non-permissive niche for intracellular bacteria during persistent Salmonella infection


ABSTRACT: Macrophages mediate key antimicrobial responses against intracellular bacterial pathogens, such as Salmonella enterica. Yet, they can also act as a permissive niche for these pathogens to persist in infected tissues within granulomas, which are immunological structures comprised of macrophages and other immune cells. We apply single-cell transcriptomics to investigate macrophage functional diversity during persistent Salmonella enterica serovar Typhimurium (STm) infection in mice. We identify determinants of macrophage heterogeneity in infected spleens and describe populations of distinct phenotypes, functional programming, and spatial localization. Using a STm mutant with impaired ability to polarize macrophage phenotypes, we find that angiotensin converting enzyme (ACE) defines a granuloma macrophage population that is non-permissive for intracellular bacteria and their abundance anticorrelates with tissue bacterial burden. Disruption of pathogen control by neutralizing TNF is linked to preferential depletion of ACE+ macrophages in infected tissues. Thus ACE+ macrophages have limited capacity to serve as cellular niche for intracellular bacteria to establish persistent infection.

ORGANISM(S): Mus musculus

PROVIDER: GSE215880 | GEO | 2022/10/20

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2013-10-07 | PXD000389 | Pride
2016-09-14 | GSE79363 | GEO
2023-05-17 | GSE215053 | GEO
2020-01-24 | PXD010179 | Pride
2022-10-19 | GSE139207 | GEO
2022-10-19 | GSE139206 | GEO
2022-10-19 | GSE139205 | GEO
2005-08-24 | E-GEOD-3179 | biostudies-arrayexpress
2024-01-25 | GSE224985 | GEO
2015-09-07 | GSE65531 | GEO