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Intranasal SIIN-Q11 induces comparable activation of OT-1 cells in Batf3KO and WT mice

ABSTRACT: To investigate if CD11b+ DCs contribute to OT-1 priming, genome transcriptome analysis was performed to compare the quality of OT-1 activation in WT or Batf3KO mice.

ORGANISM(S): Mus musculus

PROVIDER: GSE216099 | GEO | 2023/11/29

REPOSITORIES: GEO

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Intranasal SIIN-Q11 induces comparable activation of OT-1 cells in Batf3KO and WT mice

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Intranasal SIIN-Q11 induces shared transcriptional profiles in LN CD103+ and CD11b+ DCs

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Activation and presentation of SIINFEKL:Kb by lung CD11b+ and CD103+ DCs after intranasal SIIN-Q11.

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Next Generation Sequencing Facilitates Quantitative Analysis of WT and Il2ra mut/mut memory OT-I cell transcriptomes

Project description:Cognate antigen signals control CD8+ T cell priming, expansion size and effector versus memory cell fates, however, it is not clear whether they can also modulate the functional features of memory CD8+ T cells. We observed that OT-I cells that were primed with weak cognate antigen signals incorporate more cytokine signals, leading to a hypothesis that CD8+ T cells that receive weak TCR signals require cytokine signals to form functional memory. Using a previously described mouse model in which IL-2 signaling via its high affinity receptor CD25 is selectively impaired, the “Il2ramut/mut” mouse, we conducted a comparative analysis of gene expression and epigenetic landscape of Il2ramut/mut and WT OT-I memory cells that were primed with strong (Lm-Ova N4) versus weak (Lm-Ova T4). RNA seq data showed that both TCR and IL-2 priming signals have minimal effect on gene expression in resting memory CD8 T cells, but they significantly modify the epigenetic landscape of the memory CD8 T cells. These findings have important contributions to the current understanding of how priming signals program memory CD8 T cells in vivo.

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