Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MiR-29ab regulates intestinal stem cell fate through fatty-acid-oxidation-related energy metabolism [before irradiation]

ABSTRACT: To investigate the mechanism of miR-29ab in regulation of intestinal stem cells and partipation in epithelium post-injury regeneration, we established miR-29ab1-/- mice and applied irradiation to damage stem cell. We then performed gene expression profiling analysis using data obtained from RNA-seq of intestinal crypts from WT and miR-29ab1-/- mice before and after irradiation.

ORGANISM(S): Mus musculus

PROVIDER: GSE216122 | GEO | 2023/12/31

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

MiR-29ab regulates intestinal stem cell fate through fatty-acid-oxidation-related energy metabolism [after irradiation]

Project description:To investigate the mechanism of miR-29ab in regulation of intestinal stem cells and partipation in epithelium post-injury regeneration, we established miR-29ab1-/- mice and applied irradiation to damage stem cell. We then performed gene expression profiling analysis using data obtained from RNA-seq of intestinal crypts from WT and miR-29ab1-/- mice 3 day after irradiation.

2023-12-31 | GSE216669 | GEO

MiR-29ab regulates intestinal stem cell fate through fatty-acid-oxidation-related energy metabolism [before irradiation]

Project description:MiR-29ab regulates intestinal stem cell fate through fatty-acid-oxidation-related energy metabolism [before irradiation]

| PRJNA892116 | ENA

MiR-29ab regulates intestinal stem cell fate through fatty-acid-oxidation-related energy metabolism [after irradiation]

Project description:MiR-29ab regulates intestinal stem cell fate through fatty-acid-oxidation-related energy metabolism [after irradiation]

| PRJNA894863 | ENA

6 Gy gamma-irradiation of mouse small intestinal organoids at 24h, 48h and 96h after irradiation

Project description:The intact intestinal epithelial barrier protects our body from a range of immune mediated diseases. The epithelial layer has an impressive ability to reconstitute and repair upon damage and this process of repair is increasingly seen as a therapeutic target. In vitro models to study this process in primary intestinal cells are lacking. Therefore we established and characterized an in vitro model of intestinal damage and repair by applying γ-radiation on small intestinal organoids. We then used this model to identify novel regulators of intestinal regeneration. Small intestinal single crypts were isolated from C57BL/6 mice and cultured in matrigel. We treated the organoid cultures, 3 days after seeding, with 6 Gy of gamma-irradiation. RNA was isolated at 24, 48 and 96 hours after irradiation. Non-irradiated controls were collected per time point.

2019-12-06 | GSE141515 | GEO

MiR-29ab regulates intestinal stem cell fate through fatty-acid-oxidation-related energy metabolism

Project description:MiR-29ab regulates intestinal stem cell fate through fatty-acid-oxidation-related energy metabolism

| PRJNA894864 | ENA

RNA-seq analysis of crypt samples isolated from the small intestine of Bach2 KO and control mice

Project description:Epithelial regeneration is critical for barrier maintenance and organ function after intestinal injury. Here, we found that Bach2 deficiency promotes the intestinal epithelial cell proliferation during homeostasis and facilitates the crypt regeneration after irradiation, resulting in a reduction of mortality. RNA-seq analysis of isolated crypts revealed that Bach2 deficiency altered expression of numerous genes including those regulating DSBs repair. Crypts were isolated from the small intestines of Bach2 KO and control mice (n=3, respectively) 10 days after tamoxifen administration. Total RNA was extracted for sequencing using RNA-Seq.

2021-01-12 | E-MTAB-9865 | biostudies-arrayexpress

Mus musculus strain:C57BL/6J

Project description:RNA-Seq of intestinal crypts after 10Gy irradiation

| PRJDB16207 | ENA

Cycling stem cells regenerate the damaged intestinal epithelium upon irradiation

Project description:The intestinal epithelium undergoes DNA damage response, regenerative response and homeostasis after suffering irradiation. To understand the dynamic change of intestinal epithelium during the irrradiation recovery, we performed single cell RNA sequencing on irradiation-resistant +4 cells in homeostasis and its lineages at different time points after radiation.

2020-06-07 | GSE145866 | GEO

Cycling stem cells regenerate the damaged intestinal epithelium upon irradiation

Project description:Cycling stem cells regenerate the damaged intestinal epithelium upon irradiation

| PRJNA608538 | ENA

Small intestinal tissue 96h after 14 Gy whole body gamma-irradiation of VillinCreERT2 Hnf4a knockout mice

Project description:This experiment was performed to assess the effect of loss of Hnf4a in the intestinal epithelium on the regeneration process after irradiation. Intestinal epithelium specific knockout was obtained by oral adminstration of tamoxifen to VillinCreERT2+-Hnf4afl/fl mice and VillinCreERT2--Hnf4afl/fl mice (which served as controls). 3 weeks after the tamoxifen administration, mice received 14 Gy whole-body gamma-irradiation. RNA was extracted from small intestinal tissue 96 hours after irradiation.

2019-12-06 | GSE141518 | GEO