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Heterologous expression analysis of wild type Fusarium fujikuroi and Ffvel1deletion strains grown under nitrogen-limiting conditions in liquid media over time.

ABSTRACT: Investigation of whole genome gene expression level differences of Fusarium fujikuroi between wild-type and a Ffvel1 (velvet) deletion mutant in liquid medium with minimal nitrogen between 24 hr, 72 hr and 120 hr of growth using an array based on a F. verticillioides gene call set. Fusarium fujikuroi produces a number of secondary metabolites including gibberellins, bikaverin, fumonisin and fusarin C that are influenced by nitrogen availability and the velvet global regulatory complex.

ORGANISM(S): Fusarium verticillioides Fusarium fujikuroi

PROVIDER: GSE21623 | GEO | 2010/12/31

SECONDARY ACCESSION(S): PRJNA127113

REPOSITORIES: GEO

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Project description:The phytopathogenic fungus Fusarium fujikuroi is the causal agent of bakanae disease on rice due to its ability to produce gibberellins. Besides these phytohormones, F. fujikuroi is able to produce a wide range of other secondary metabolites (SMs), such as mycotoxins and pigments. Although much progress has been made in the field of secondary metabolism over the last years, the transcriptional regulation of SM biosynthetic genes is complex and far from being fully understood. Environmental conditions (e.g. nitrogen availability and pH), global and pathway-specific regulators as well as chromatin remodeling were shown to play major roles in this regulation. Here, the role of FfSge1, a homolog of the morphological switch regulators Wor1 and Ryp1 in Candida albicans and Histoplasma capsulatum, respectively, is explored with emphasis on secondary metabolism. FfSge1 is not required for conidia formation and pathogenicity, but is involved in vegetative growth. Genome-wide transcriptome analysis of the M-NM-^Tffsge1 deletion mutant compared to the wild type revealed that FfSge1 is a global regulator of secondary metabolism in F. fujikuroi that activates the expression of several SMs. In addition, FfSge1 is also required for expression of a yet uncharacterized SM gene cluster containing a noncanonical non-ribosomal peptide synthetase. Investigation of whole genome gene expression of the Fusarium fujikuroi wild type IMI58289, M-NM-^Tffsge1 mutant under nitrogen starvation and nitrogen sufficient conditions. In this study we hybridized in total 8 microarrays using total RNA recovered from a wild-type culture of F. fujikuroi IMI58289 and M-NM-^Tffsge1 mutant culture. All cultures were grown on a 6 mM Gln (10%) and a 60 mM Gln medium (100%). For each combination of culture and medium a biological replicate was created. Each chip measures the expression level of 14,397 genes from F. fujikuroi IMI58289 with eight 60-mer probes. Please note that the wild type samples have been published as part of the GEO accession GSE43745, but re-analyzed with the M-NM-^Tffsge1 mutant samples in the current study.

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Heterologous expression analysis of wild type Fusarium fujikuroi and Ffvel1deletion strains grown under nitrogen-limiting conditions in liquid media over time.

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