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Characterization of human pluripotent stem cell differentiation by single-cell dual-omics analyses (scATAC-seq)

ABSTRACT: In vivo differentiation of human pluripotent stem cells (hPSCs) has unique advantages such as multilineage differentiation, angiogenesis, and close cell‒cell interactions. To systematically investigate the multilineage differentiation mechanisms of hPSCs, we constructed the in vivo hPSC differentiation landscape containing 239,670 cells using teratoma models. We inferred 18 cell differentiation trajectories and characterized common and specific gene regulation patterns during hPSC differentiation at both transcriptional and epigenetic levels. The results of developing single cell Basic Local Alignment Search Tool (dscBLAST), our self-developed tool for cell identification and developmental stage speculation, further confirmed the differentiated cell identity and suggested that cells in the teratoma are largely at immature developmental stages. Overall, our study offers new insights into stem cell fate decisions and embryonic development; dscBLAST shows favorable cell identification performance, providing both developmental and adult cells with a powerful tool for cell annotation and stage speculation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE216323 | GEO | 2022/10/28

REPOSITORIES: GEO

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Characterization of human pluripotent stem cell differentiation by single-cell dual-omics analyses (scRNA-seq)

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