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The study of SOX2 deposition in asynchronous and mitotic E14TG2a mouse ES cells.

ABSTRACT: We executed CUT&RUN-seq for SOX2 a different mouse strain E14TG2a

ORGANISM(S): Mus musculus

PROVIDER: GSE216659 | GEO | 2023/03/19

REPOSITORIES: GEO

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The study of SOX2 deposition in asynchronous and mitotic E14TG2a cells

Project description:We executed ChIPseq for SOX2 a different mouse strain E14TG2a.

2023-03-19 | GSE216663 | GEO

The study of SOX2 deposition in asynchronous and mitotic E14TG2a mouse ES cells.

Project description:The study of SOX2 deposition in asynchronous and mitotic E14TG2a mouse ES cells.

| PRJNA894706 | ENA

The study of SOX2 deposition in asynchronous and mitotic E14TG2a cells

Project description:The study of SOX2 deposition in asynchronous and mitotic E14TG2a cells

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The study of chromatin occupancy other mitotically bound factors upon the mitotic depletion of SMARCE1 in mouse ES cells.

Project description:We executed CUT&RUN-seq for SOX2, ESRRB, and EZH2 upon the mitotic depletion of SMARCE1 in mouse ES cells.

2023-03-19 | GSE216661 | GEO

The study of SWI/SNF subunits and other transcription factors deposition in asynchronous and mitotic mESCs and cells released from mitosis

Project description:We executed CUT&RUN-seq for SWI/SNF components ARID1A, BRD9, SMARCA4, SMARCB1, SMARCE1, as well as ESRRB, SOX2, and EZH2 in asynchronous and mitotic cells and reported that, in asynchronous cells, ARID1A localized primarily at enhancer regions and EZH2 preferentially deposited at bivalent promoters and silent enhancer domains. The remaining factors were enriched at both TSS/promoters and to varying degrees at active enhancers. Unexpectedly, in mitosis, the chromatin regulatory factors almost all tethered at proximal gene regions with very little binding at enhancers. While the SWI/SNF subunits were bound principally at promoters, EZH2, the catalytic subunit of Polycomb Repressive Complex 2 was bound at both promoters and silent enhancers in mitotic cells. Moreover, we reported that upon the degradation of SMARCE1 in mitosis, the occupancy of SOX2, ESRRB, and EZH2 on mitotic chromatin was disrupted.

2023-03-19 | GSE189560 | GEO

The study of SMARCE1 and SOX2 deposition in DMSO or BRM014 treated Smarce1-MD(R42A) and Smarce1-MD cells at 90 min after releasing from mitosis.

Project description:We executed CUT&RUN-seq for SMARCE1 and SOX2 in BRG1 inhibitor BRM014 treated ,in MD or MD mutatnt mouse embryonic stem cells at 90 min from mitotic release.

2023-03-19 | GSE216657 | GEO

Mus musculus isolate:ES-E14TG2a

Project description:Mus musculus isolate:ES-E14TG2a Raw sequence reads

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Genome-wide analysis of SOX2 binding in asynchronous and mitotic mouse embryonic stem cells.

Project description:SOX2 is part of the core network of transcription factors regulating embryonic stem cell pluripotency. We found that SOX2 has the ability to remain bound to mitotic chromosomes, in contrast to most transcription factors that are excluded from mitotic chromatin as transcription shuts down. We obtained a highly purified population of mitotic mouse embryonic stem cells and compared the genome-wide binding profile of SOX2 to that in asynchronous cells by Chromatin Immunoprecipitation followed by high throughput sequencing (ChIP-seq), and show that SOX2 remains bound to a small set of genes during mitosis.

2016-11-07 | GSE89599 | GEO

The study of histone modifications and SOX2 occupancy in asynchronous and mitotic mESCs.

Project description:We performed ChIP-seq for H3K4me3, H3K27ac, H3K4me1, H3K27me3, H3K36me3, H3K9me3, and H4K20me3 to characterize chromatin states and investigated SOX2 deposition in asynchronous and mitotic cells.

2023-03-19 | GSE189561 | GEO

ATAC-seq on mouse embryonic stem cells for asynchronous cells, mitotic cells and purified mitotic chromosomes.

Project description:E14Tg2a mouse embryonic stem cells (ESCs) were cultured on gelatin coated plates in KO-DMEM medium supplemented with 15% FCS and 1000 U/ml leukaemia inhibitory factor. Rapidly dividing cultures of ESCs were arrested in metaphase using demecolcine (0.1 μg/ml for 6 h), achieving samples where most (85-90%) cells were in M-phase as judged by propidium iodide labelling. Condensed chromosomes were released using polyamine buffer, stained with Hoechst 33258 and Chromomycin A3 and purified by flow cytometry using a Becton Dickinson Influx equipped with specialised air-cooled lasers.

2020-06-14 | GSE147552 | GEO

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