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Gene expression in glucose-grown L-pipecolic acid production strains C.glutamicum LPA-1A and LPA-5 compared to the non-L-pipecolic acid-producing strain C. glutamicum LPA-0 [2]

ABSTRACT: mRNA was sampled during exponential growth (OD660 = 4) strains: LPA-0 (L-lysine hyperproducing strain C. glutamicum LYS-12 (Becker et al. 2011) + pClik5a_MCS), C. glutamicum LPA-5 (C. glutamicum LPA-0 + pClik5a_peftu_lat_proC)

ORGANISM(S): Corynebacterium glutamicum

PROVIDER: GSE216735 | GEO | 2024/01/01

REPOSITORIES: GEO

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Project description:The Gram-positive soil bacterium Corynebacterium glutamicum is widely used in industrial fermentative processes for the production of amino acids. The world production of L-lysine has surpassed 2 million tons per year. Glucose is taken up into the C. glutamicum cell by the phosphotransferase system PTS which can be replaced and/or enhanced by a permease and a glucokinase. Heterologous expression of the gene for the high-affinity glucose permease from Streptomyces coelicolor and of the Bacillus subitilis glucokinase gene fully compensated for the absence of the PTS in ï??hpr strains and strains grew as fast with glucose as C. glutamicum wild type. Growth of PTS-positive strains with glucose was accelerated when the endogenous inositol permease IolT2 and the glucokinase from Bacillus subtilis were overproduced using plasmid pEKEx3-IolTBest. When the genome-reduced C. glutamicum strain GRLys1 carrying additional in-frame deletions of sugR and ldhA to derepress glycolytic and PTS genes and to circumvent formation of L-lactate as by-product was transformed with this plasmid, a 40% higher L-lysine titer and a 30% higher volumetric productivity as compared to GRLys1(pEKEx3) resulted. The non-proteinogenic amino acid pipecolic acid (L-PA), a precursor of immunosuppressants, peptide antibiotics or piperidine alkaloids, can be derived from L-lysine. To enable production of L-PA by the L-lysine producing strain, the L-Lysine dehydrogenase gene lysDH from Silicibacter pomeroyi and the endogenous pyrroline 5-carboxylate reductase gene proC were expressed as synthetic operon. This enabled C. glutamicum to L-PA with a yield of 0.49 Â± 0.03 gg-1 and a volumetric productivity of 0.04 Â± 0.00 gL-1h-1.To the best of our knowledge, this is the first fermentative process for the production of L-PA. Two conditions tested, 200 mM NaCl Vs 200 mM pipecolic supplemented in the culture medium, control experiments done with the addition of 200mM of NaCl. Four technical replicates.

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Gene expression in glucose-grown L-pipecolic acid production strains C.glutamicum LPA-1A and LPA-5 compared to the non-L-pipecolic acid-producing strain C. glutamicum LPA-0 [2]

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