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Multiple genes of Candida albicans influencing echinocandin susceptibility in caspofungin-adapted mutants

ABSTRACT: Expression profiles of five caspofungin adapted candida albicans strains were analysed from their parental strains

ORGANISM(S): Candida albicans

PROVIDER: GSE217094 | GEO | 2022/11/05

REPOSITORIES: GEO

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Multiple Genes of Candida albicans Influencing Echinocandin Susceptibility in Caspofungin-Adapted Mutants.

Project description:Candida albicans is an opportunistic human fungal pathogen that causes invasive infections in immunocompromised individuals. Despite the high anticandidal activity among the echinocandins (ECNs), a first-line therapy, resistance remains an issue. Furthermore, many clinical isolates display decreased ECN susceptibility, a physiological state which is thought to lead to resistance. Determining the factors that can decrease susceptibility is of high importance. We searched for such factors genome-wide by comparing the transcriptional profiles of five mutants that acquired decreased caspofungin susceptibility in vitro in the absence of canonical FKS1 resistance mutations. The mutants were derived from two genetic backgrounds and arose due to independent mutational events, some with monosomic chromosome 5 (Ch5). We found that the mutants exhibit common transcriptional changes. In particular, all mutants upregulate five genes from Ch2 in concert. Knockout experiments show that all five genes positively influence caspofungin and anidulafungin susceptibility and play a role in regulating the cell wall mannan and glucan contents. The functions of three of these genes, orf19.1766, orf19.6867, and orf19.5833, were previously unknown, and our work expands the known functions of LEU42 and PR26. Importantly, orf19.1766 and LEU42 have no human orthologues. Our results provide important clues as to basic mechanisms of survival in the presence of ECNs while identifying new genes controlling ECN susceptibility and revealing new targets for the development of novel antifungal drugs.

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Multiple genes of Candida albicans influencing echinocandin susceptibility in caspofungin-adapted mutants

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Project description:Approximately 200 cells of Candida albicans lab strain SC5314 were spread on YPD plates supplemented with 2 fold increase from 50 ng/ml to 12800ng/ml caspofungin. All adaptors appeared were collected. Randomly 36 tolerant adaptors were sequenced.

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Gcn5 Controls Virulence of the Human Fungal Pathogen Candida albicans through Multiple Regulatory Networks

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Whole genome sequencing of caspofungin adaptors derived from homozygous deletion strains of PKC and calcineurin pathways in Candida albicasn

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Caspofungin-induced Responses in the Planktonic Cells and Biofilms of Candida albicans

Project description:This project investigated the signature profiles of C. albicans planktonic cells and biofilms in response to caspofungin.

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Transcriptome profile of tra1 mutant in C. albicans in response to the antifungal caspofungin

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<i>Candida albicans</i> Genes Modulating Echinocandin Susceptibility of Caspofungin-Adapted Mutants Are Constitutively Expressed in Clinical Isolates with Intermediate or Full Resistance to Echinocandins.

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Whole genome sequencing of caspofungin adaptors in Candida albicans

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Candida albicans and Candida glabrata clinical isolates exhibiting reduced echinocandin susceptibility.

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