Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcritome analysis of HeLa cells under ER or metabolic stress

ABSTRACT: To investigate functional ncRNAs in response to cellular stresses, we performed high-throughput RNA sequencing (RNA-seq) of HeLa cells under ER or metabolic stresses.

ORGANISM(S): Homo sapiens

PROVIDER: GSE217305 | GEO | 2022/12/02

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcritome analysis of HeLa cells under ER or metabolic stress

Project description:Transcritome analysis of HeLa cells under ER or metabolic stress

| PRJNA898373 | ENA

ERalpha ChIP-chip from HeLA-ER cells treated with E2

Project description:We are studying the mechanisms by which the estrogen receptor, ERalpha, is recruited to and regulates genes with a non-direct DNA binding. We performed ChIP-chip for ERalpha in E2 treated HeLa-ER cells, and looked at 19000 RefSeq genes to determine binding patterns of the receptor at promoters. The experiment was performed in duplicate. ChIP-chip biological replicates for Eralpha in E2 treated HeLa-ER cells are included.

2011-02-22 | E-GEOD-22099 | biostudies-arrayexpress

ERalpha ChIP-chip from HeLA-ER cells treated with E2

2011-02-22 | GSE22099 | GEO

Quantitative ER chemical proteomics with ER-localizable reactive molecules under ER-stress condition

Project description:The endoplasmic reticulum (ER) is an organelle that performs a variety of essential cellular functions via interactions with other organelles. Despite its important role, chemical tools for profiling the composition and dynamics of ER proteins remain very limited because of the labile nature of these proteins. Here, we developed ER-localizable reactive molecules (called ERMs) as tools for ER-focused chemical proteomics. ERMs can spontaneously localize in the ER of living cells and selectively label ER-associated proteins with a combined affinity and imaging tag, enabling tag-mediated ER protein enrichment and identification with liquid chromatography tandem-mass spectrometry (LC-MS/MS). The ERM probes could be used simultaneously with the nucleus- and mitochondria-localizable reactive molecules previously developed by our group, which enabled orthogonal organellar chemoproteomics in a single biological sample. Moreover, quantitative analysis of the dynamic changes in ER-associated proteins in response to tunicamycin-induced ER stress was performed by combining ER-specific labeling with SILAC (stable isotope labeling by amino acids in cell culture)-based quantitative MS technology. Our results demonstrated that ERM-based chemical proteomics provides a powerful tool for labeling and profiling ER-related proteins in living cells.

2018-11-22 | PXD011619 | Pride

Endoplasmic reticulum (ER) stress and the unfolded protein response (UPR)

Project description:We are currently studying how these information-carrying oligosaccharides are involved in cellular stress responses, particularly in human genetic diseases called Congenital Disorders Of Glycosylation in which oligosaccharide assembly is defective. We are interested in endoplasmic stress responses/unfolded protein responses. ER glycans and ER lectins are intimately involved in the folding of ER proteins. Therefore, ER lectins and ER glycosyltransferases may be controlled by these stress responses, to produce and/or recognize key glycans in the stress response. Our model system is the human dermal fibroblast. We have successfully calibrated the ER stress responses in these cells by determining the magnitudes of various stress effects (such as chaperone transcription) with different forms of ER stress. We are also completing a study in which activation the signaling molecules Ire1 and ATF6 are also calibrated. We prepared multiple identical aliquots of RNA from cells stressed under several of these calibrated conditions. Endoplasmic reticulum (ER) stress and the unfolded protein response (UPR): Study of the role of ER stress in the expression of genes for ER transferases and lectins that participate in ER folding and quality control using human skin fibroblasts. The UPR response in these cells has been calibrated using various readouts in response to different stresses: 1) 40 nM L-azetidine-2-carboxylate (AZC) 1 hour, 2) 0.2 mg/ml castanospermine, 24 hours, 3) 2 mM Dithiothreitol (DTT), 20 minutes, 4) 100nm Thapsigargin (TG), 30 minutes, 5) 5 ug/ml tunicamycin, 5 hours, 6) untreated control cells

2011-02-17 | E-GEOD-27349 | biostudies-arrayexpress

G3BP2B stress granules regulate mRNA expression under ER stress [2]

Project description:G3BP2B stress granules regulate mRNA expression under ER stress [2]

| PRJNA1071167 | ENA

G3BP2B stress granules regulate mRNA expression under ER stress [3]

Project description:G3BP2B stress granules regulate mRNA expression under ER stress [3]

| PRJNA1071168 | ENA

G3BP2B stress granules regulate mRNA expression under ER stress [1]

Project description:G3BP2B stress granules regulate mRNA expression under ER stress [1]

| PRJNA1071166 | ENA

G3BP2B stress granules regulate mRNA expression under ER stress [Riboseq_Exp1]

Project description:G3BP2B stress granules regulate mRNA expression under ER stress [Riboseq_Exp1]