Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Role of PARP-1 and PR-Set7 in transcriptional regulation during development (RNA-seq)

ABSTRACT: We interrogated the role of PARP-1 and PR-Set7 in developmental gene regulation in Drosophila via RNA-seq in third-instar larvae.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE217728 | GEO | 2023/05/25

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Role of PARP-1 in transcriptional regulation during development (ChIP-seq)

Project description:We interrogated the role of PARP-1 in developmental gene regulation in Drosophila via ChIP-seq in third-instar larvae.

2023-05-25 | GSE217729 | GEO

Differential binding of PARP-1 domains (AW-YFP ChIP-seq)

Project description:We interrogated the differential binding of PARP-1 domains (ZnI, ZnII, AD-WGR) and a ZnI mutant in Drosophila via ChIP-seq of third-instar larvae.

2023-05-25 | GSE222876 | GEO

Differential binding of PARP-1 domains (ZNII-YFP ChIP-seq)

Project description:We interrogated the differential binding of PARP-1 domains (ZnI, ZnII, AD-WGR) and a ZnI mutant in Drosophila via ChIP-seq of third-instar larvae.

2023-05-25 | GSE222875 | GEO

Differential binding of PARP-1 domains (ZNI-YFP ChIP-seq)

Project description:We interrogated the differential binding of PARP-1 domains (ZnI, ZnII, AD-WGR) and a ZnI mutant in Drosophila via ChIP-seq of third-instar larvae.

2023-05-25 | GSE222873 | GEO

Differential binding of PARP-1 domains (DZNI-YFP ChIP-seq)

Project description:We interrogated the differential binding of PARP-1 domains (ZnI, ZnII, AD-WGR) and a ZnI mutant in Drosophila via ChIP-seq of third-instar larvae.

2023-05-25 | GSE222865 | GEO

Histone lysine methyltransferase Pr-set7/SETD8 promotes neural stem cell reactivation

Project description:The ability of neural stem cells (NSCs) to switch between quiescence and proliferation is crucial for brain development and homeostasis. Increasing evidence suggest that variants of histone lysine methyltransferases including KMT5A are associated with neurodevelopmental disorders. However, the function of KMT5A/Pr-set7/SETD8 in the central nervous system is not well established. Here, we show that Drosophila Pr-Set7 is a novel regulator of NSC reactivation. Loss-of-function of pr-set7 causes a delay in NSC reactivation and loss of H4K20 monomethylation in the brain. Through NSC-specific in vivo profiling, we demonstrate that Pr-set7 binds to the promoter region of cyclin dependent kinase 1 (cdk1) and Wnt pathway transcriptional co-activator earthbound1/jerky (ebd1). Further validation indicates that Pr-set7 is required for the expression of cdk1 and ebd1 in the brain. Similar to Pr-set7, Cdk1 and Ebd1 promote NSC reactivation. Moreover, Cdk1 upregulates the Ebd1 levels in NSCs, while Ebd1 appears to downregulate Cdk1 expression, suggesting a negative feedback regulation. Finally, overexpression of Cdk1 and Ebd1 significantly suppressed NSC reactivation defects observed in pr-set7-depleted brains. Therefore, Pr-set7, the sole H4K20 methyltransferase, promotes NSC reactivation through regulating Wnt signaling and cell-cycle progression. Given the conservation of Pr-set7, our findings may contribute to the understanding of mammalian KMT5A/PR-SET7/SETD8 in NSC proliferation and associated neurodevelopmental disorders.

2021-02-09 | GSE158764 | GEO

Gene expression in the mTSCs of WT and Pr-set7 KO mTSCs

Project description:To detect the gene profiles in WT and Pr-set7 KO mTSCs, mTSCs are collected and subjected to RNA-Seq. After aligned to mouse mm10 by HISAT2, RPKM value was calculated by Edger. Our results show that Pr-set7 as the key regulator for mTSCs. There are also some genes differentially expressed after Pr-set7 KO, some of the DEGs were further confirmed by qPCR, the DEGs were associated with viral mimic inflammatory activities, antiviral innate immune response, genomic instability, and programmed cell death, indicating the essential role of Pr-set7. This RNA-Seq data provides fundamental information for our further physiological study of Pr-set7.

2023-06-28 | GSE196224 | GEO

Gene expression in the hTSCs of si-NC and si-PR-SET7 KD hTSCs

Project description:To detect the gene profiles in si-NC and si-PR-SET7 KD hTSCs, hTSCs are collected and subjected to RNA-Seq. After aligned to mouse hg38 by HISAT2, RPKM value was calculated by Edger. Our results show that PR-SET7 as the key regulator for hTSCs. There are also some genes differentially expressed after PR-SET7 KD, some of the DEGs were further confirmed by qPCR, the DEGs were associated with viral mimic inflammatory activities, antiviral innate immune response, genomic instability, and programmed cell death, indicating the essential role of PR-SET7. This RNA-Seq data provides fundamental information for our further physiological study of PR-SET7.

2023-06-28 | GSE196223 | GEO

The UBC9 E2 SUMO conjugating enzyme binds the PR-Set7 histone methyltransferase to facilitate target gene repression

Project description:To detect genes whose expression is signigicantly altered upon depletion of pr-SET7 Total RNA obtained from 4 days post-transfection of control pSuperior empty plasmid and pSuperior pr-SET7 shRNA plasmid

2011-07-02 | E-GEOD-30361 | biostudies-arrayexpress

Role of PARP-1 and PR-Set7 in transcriptional regulation during development (RNA-seq)

Project description:Role of PARP-1 and PR-Set7 in transcriptional regulation during development (RNA-seq)

| PRJNA900231 | ENA