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Optimization of chondrocyte isolation from human articular cartilage to preserve the chondrocyte transcriptome [run92]

ABSTRACT: The isolation of chondrocytes from human articular cartilage for single-cell RNA sequencing requires extensive and prolonged tissue digestion at 37 °C. Modulations of the transcriptional activity likely take place during this period such that the transcriptomes of isolated human chondrocytes no longer match their original status in vivo. Here, we optimized the human chondrocyte isolation procedure to maximally preserve the in vivo transcriptome.

ORGANISM(S): Homo sapiens

PROVIDER: GSE217870 | GEO | 2022/11/15

REPOSITORIES: GEO

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Optimization of chondrocyte isolation from human articular cartilage to preserve the chondrocyte transcriptome [run54]

Project description:The isolation of chondrocytes from human articular cartilage for single-cell RNA sequencing requires extensive and prolonged tissue digestion at 37 °C. Modulations of the transcriptional activity likely take place during this period such that the transcriptomes of isolated human chondrocytes no longer match their original status in vivo. Here, we optimized the human chondrocyte isolation procedure to maximally preserve the in vivo transcriptome.

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Optimization of chondrocyte isolation from human articular cartilage to preserve the chondrocyte transcriptome

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Optimization of chondrocyte isolation from human articular cartilage to preserve the chondrocyte transcriptome [run54]

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Optimization of chondrocyte isolation from human articular cartilage to preserve the chondrocyte transcriptome [run92]

Project description:Optimization of chondrocyte isolation from human articular cartilage to preserve the chondrocyte transcriptome [run92]

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DNA methyltransferase 3b regulates articular cartilage homeostasis by altering chondrocyte metabolism

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Optimization of chondrocyte isolation from human articular cartilage to preserve the chondrocyte transcriptome

Project description:This SuperSeries is composed of the SubSeries listed below.

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