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Precision RNAi using synthetic shRNAmir target sites

ABSTRACT: Loss-of-function genetic tools are widely applied for validating therapeutic targets, but their utility remains limited by incomplete on- and uncontrolled off-target effects. We describe artificial RNA interference (ARTi) based on synthetic, ultra-potent, off-target-free shRNAs that enable efficient, inducible, and reversible suppression of any gene upon introduction of a synthetic target sequence into non-coding transcript regions. ARTi establishes a scalable loss-of-function tool with full control over on- and off-target effects.

ORGANISM(S): Homo sapiens

PROVIDER: GSE218404 | GEO | 2023/07/04

REPOSITORIES: GEO

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