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Establish a rapid and efficient chemical reprogramming platform [scRNA-Seq]

ABSTRACT: Chemically reprogramming somatic cells to iPSCs is time-consuming and low-efficiency. Here, we discribe a rapid chemical reprogramming condition enabling generating iPSCs from MEFs in 12 days. To further investigate the mechemisms and draw an epigenetic maps during the rapid chemical reprogramming, we performed time-course RNA-seq, ATAC-seq, RRBS, CUT&Tag (H3K4me3, H3K18la, H3K9me3, H3K27me3 and H3K27ac) and scRNA-seq.

ORGANISM(S): Mus musculus

PROVIDER: GSE218855 | GEO | 2023/05/03

REPOSITORIES: GEO

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