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RNA-seq transcriptional profiling in the Fah-/- livers after transplantation and YC treatment

ABSTRACT: The transplanted mature hepatocytes were found to dedifferentiate into hepatic progenitor cells (HPCs), which proliferate and then convert back to mature state at the completion of liver repopulation. The combination of two small molecules Y-27632 (Y, Rock inhibitor) and CHIR99021 (C, Wnt agonist) could convert mouse primary hepatocytes into HPCs, which could be passaged for more than 30 passages in vitro. Moreover, YC could stimulate the proliferation of transplanted hepatocytes in Fah-/- liver by promoting the conversion into HPCs. Netarsudil (N) and LY2090314 (L), two clinically used drugs which target the same pathways as YC, could also promote hepatocytes proliferation in vitro and in vivo, by facilitating HPC conversion.

ORGANISM(S): Mus musculus

PROVIDER: GSE218952 | GEO | 2022/12/17

REPOSITORIES: GEO

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