Transcriptomics

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CRISPR-based epigenome editing screens identify transcriptional and epigenetic regulators of human CD8 T cell function [CRISPRi/a TF scRNA-seq characterization]


ABSTRACT: Human CD8+CCR7+ T cells from three donors were transduced with CRISPRi and CRISPRa TF gRNA libraries and expanded for 10 days. After 10 days, we used single-cell RNA sequencing (scRNA-seq) to analyze the transcriptomic effects of silencing or activating each gene candidate.

ORGANISM(S): Homo sapiens

PROVIDER: GSE218985 | GEO | 2023/05/24

REPOSITORIES: GEO

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