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Phenotype of stim2 zebrafish knockout

ABSTRACT: Stromal interaction molecules (STIMs) are endoplasmic reticulum resident proteins that regulate Ca2+ homeostasis and signaling by store-operated calcium entry (SOCE). The different properties and functions of STIM1 and STIM2 have been described mostly based on work in vitro. Since STIM2 knockout mice do not survive till the adulthood we generated stim2a and stim2b double knockout zebrafish and characterized it. This (stim2a;stim2b)-/- fish were fertile and did not have apparent morphological phenotype. However, RNA-sequencing revealed 1424 genes differentially expressed with at least 2-fold change. One of the most upregulated gene was annexinA3a, which is a marker of activated microglia. This corresponded well to augmented Neutral Red staining observed by imaging in vivo of (stim2a;stim2b)-/- brain. The lack of Stim2 decreased survival of fish under low oxygen conditions. Behavioral tests such as visual-motor response and dark-light preferences indicated that (stim2a;stim2b)-/- larvae might have problems with vision. This is consistent with GO BP's analysis, which showed downregulation of many genes related to light perception. The Periodic acid-Schiff staining of retina sections from adult fish reveled the alteration in stratum pigmentosum suggesting involvement of Stim2-dependent process in visual perception. Together, these data reveal new functions for Stim2 in nervous system.

ORGANISM(S): Danio rerio

PROVIDER: GSE219273 | GEO | 2024/12/31

REPOSITORIES: GEO

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