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Expression data from Caco-2 cells co-cultivated with B. animalis subsp. lactis BB-12

ABSTRACT: Although intestinal microbiota play a pivotal role in the development of host immune system this biological issue was not so far studied in great detail. In this study we examined immune response of Caco-2 enterocytes after incubation with common probiotic Bifidobacterium animalis subsp. lactis BB-12 for 4 hours. We used microarrays to inspect the global gene expression of Caco-2 cells upon co-culturing with B. animalis subsp. lactis BB-12 and several distinct immune-related genes up-regulated during this process.

ORGANISM(S): Homo sapiens

PROVIDER: GSE21930 | GEO | 2010/07/01

SECONDARY ACCESSION(S): PRJNA126869

REPOSITORIES: GEO

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Expression data from Caco-2 cells co-cultivated with B. animalis subsp. lactis BB-12

Project description:Although intestinal microbiota play a pivotal role in the development of host immune system this biological issue was not so far studied in great detail. In this study we examined immune response of Caco-2 enterocytes after incubation with common probiotic Bifidobacterium animalis subsp. lactis BB-12 for 4 hours. We used microarrays to inspect the global gene expression of Caco-2 cells upon co-culturing with B. animalis subsp. lactis BB-12 and several distinct immune-related genes up-regulated during this process. One time point (T4) and two controls (T0) were analysed. T0 represent differentiated Caco-2 cells cultivated for 3 weeks. T4 represents differentiated Caco-2 cells cultivated for 3 weeks plus consequent 4 hours of co-cultivation with B. animalis subsp. lactis BB-12. 3 technical replicates for T0-1, T0-2 or T4 were pooled to a single sample, RNA extracted and further used in gene expression experiments.

2010-07-30 | E-GEOD-21930 | biostudies-arrayexpress

Bifidobacterium animalis subsp. lactis BB-12

Project description:Bifidobacterium animalis subsp. lactis BB-12 Genome sequencing and assembly

| PRJNA417226 | ENA

Transcriptome analysis of Bifidobacterium animalis subsp. lactis BB12 grown on xylo-oligosaccharides

Project description:Comparison of the growth of Bifidobacterium animalis subsp. lactis BB12 in MRS (without carbon source) with either 2% XOS (xylo-oligosaccharides) or 2% glucose using whole-genome transcriptome analysis.

2010-09-10 | GSE20322 | GEO

Bifidobacterium animalis subsp. lactis BB-12

Project description:Bifidobacterium animalis subsp. lactis genome sequencing

| PRJNA42883 | ENA

Genes involved in synthesis of lipoteichoic acids are induced when Bifidobacterium animalis subsp. lactis BB12 is incubated with Human Enterocyte-Like Caco-2 Cells

Project description:Bifidobacterium animalis subsp. lactis BB12 is a widely used probiotic bacterium. However the molecular mechanism by which this bacterium confers positive health effects to the host is largely unknown. The importance of lipoteichoic acids (LTA) in the cross-talk between the bacteria and human enterocyte-like Caco-2 cells was investigated in the present study. A microarray-based analysis of gene expression revealed induction of genes encoding enzymes involved in the synthesis of lipoteichoic acids of BB12. This observation was confirmed in phenotypic experiments, where a specific galactofuranosyl carbohydrate epitope present in the structure of lipoteichoic acids of BB12 was used as a detection marker. An exopolysaccharide EPS/LTA extract isolated from BB12 and commercially available pure LTAs from the human pathogen S. pyogenes were tested in competitive adhesion experiments. The adhesion of B. animalis subsp. lactis BB12 to Caco-2 cells was inhibited in presence of LTA. The strongest inhibition was observed in the concentration range 0.5-10 ug/ml for LTAs from S. pyogenes and 5-10 ug/ml for EPS/LTA extract from BB12. The inhibitory concentrations in both cases likely reflect the physico-chemical properties of LTAs with the highest efficacy below the critical micelle concentration, and therefore we ascribe the main inhibitory effect to LTAs for the BB12 isolated EPS/LTA extract as well. In conclusion we present increased biosynthesis of lipoteichoic acids of BB12 co-incubated with human Caco-2 cells and evidence that LTA serve as important cell wall factors involved in adhesion to these human enterocyte-like cells.

2012-08-01 | GSE21588 | GEO

Expression data from Caenorhabditis elegans fed with the bacterial strain Bifidobacterium animalis sbsp. lactis CECT 8145

Project description:Modulation of gut microbiota through probiotic supplementation is an interesting strategy to prevent obesity We use microarrays to study the global genome expression of C. elegans fed with the probiotic strain Bifidobacterium animalis sbsp. lactis CECT 8145 Wild type strain N2 of C. elegans was cutured in Nematode Growth medium (NGM, control fed) or NGM with a bacterial lawn fed of the strain B. animalis subsp. lactis CECT 8145, until reach young adult stage. Worm population were age-synchronized. RNA was isolated from each populations (control and treated) using RNAasy Kit (Qiagen) and hybridizated on Affymetrix microarrays.

2015-11-22 | E-GEOD-63531 | biostudies-arrayexpress

Genes involved in synthesis of lipoteichoic acids are induced when Bifidobacterium animalis subsp. lactis BB12 is incubated with Human Enterocyte-Like Caco-2 Cells

2012-07-31 | E-GEOD-21588 | biostudies-arrayexpress

Homo sapiens

Project description:Expression data from Caco-2 cells co-cultivated with B. animalis subsp. lactis BB-12

| PRJNA126869 | ENA

Bifidobacterium animalis subsp. lactis BB-12

Project description:Genes involved in synthesis of lipoteichoic acids are induced when Bifidobacterium animalis subsp. lactis BB12 is incubated with Human Enterocyte-Like Caco-2 Cells

| PRJNA127031 | ENA

Transcriptional and functional analysis of Bifidobacterium animalis subsp. lactis exposure to tetracycline

Project description:Commercial probiotic bacteria must be tested for acquired antibiotic resistance elements to avoid potential transfer to pathogens. The European Food Safety Authority recommends testing resistance using microdilution culture techniques previously used to establish inhibitory thresholds for the Bifidobacterium genus. Many Bifidobacterium animalis subsp. lactis strains exhibit increased resistance to tetracycline, historically attributed to the ribosomal protection gene tet(W). However, some strains that harbor genetically identical tet(W) genes show various inhibition levels suggesting that other genetic elements also contribute to observed differences. Here, we adapted several molecular assays to confirm the inhibition of B. animalis subsp. lactis strains Bl-04 and HN019, and employed RNA-seq to assess the transcriptional differences related to genomic polymorphisms. We detected specific stress responses to the antibiotic by correlating ATP concentration to viable genome copies from droplet digital PCR, and found that the bacteria were still metabolically active in high drug concentrations. Transcriptional analyses revealed that several polymorphic regions, particularly a novel multi-drug efflux transporter, were differentially expressed between the strains in each experimental condition, likely having phenotypic effects. We also found that the tet(W) gene was up-regulated only during sub-inhibitory tetracycline concentrations, while two novel tetracycline resistance genes were up-regulated at high concentrations. Furthermore, many genes involved in amino acid metabolism and transporter function were up-regulated while genes for complex carbohydrate utilization, protein metabolism, and CRISPR-Cas systems were down-regulated. These results provide high-throughput means for assessing antibiotic resistance and determine the genetic network that contributes to the global tetracycline response between two highly related probiotic strains.

2018-09-27 | GSE117878 | GEO

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