Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Microarray profiling of expression patterns of fibroblasts from different skin types

ABSTRACT: Adult human fibroblasts derived from the dermis of 4 mm punch biopsies taken from the lower backs of 15 healthy subjects of 3 different phototypes (types I, III and VI). This study was approved by the Human Research Ethics Committee of Hamburg. Subjects with type I skin were #4, 12, 15, 19 and 26; subjects with type III skin were # 6, 9, 13, 14 and 23, and subjects with type VI skin were # 7, 8, 16, 17 and 28. The fibroblasts were cultured from the biopsies and were grown in monolayer culture in high glucose Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine plus 1% penicillin and streptomycin at 37°C in a humidified 5% CO2 atmosphere. Fibroblasts were subcultured using routine methods and were used at passages 3 to 6.

ORGANISM(S): Homo sapiens

PROVIDER: GSE22022 | GEO | 2010/08/31

SECONDARY ACCESSION(S): PRJNA127443

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Microarray profiling of expression patterns of fibroblasts from different skin types

Project description:Adult human fibroblasts derived from the dermis of 4 mm punch biopsies taken from the lower backs of 15 healthy subjects of 3 different phototypes (types I, III and VI). This study was approved by the Human Research Ethics Committee of Hamburg. Subjects with type I skin were #4, 12, 15, 19 and 26; subjects with type III skin were # 6, 9, 13, 14 and 23, and subjects with type VI skin were # 7, 8, 16, 17 and 28. The fibroblasts were cultured from the biopsies and were grown in monolayer culture in high glucose Dulbecco’s modified Eagle medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% L-glutamine plus 1% penicillin and streptomycin at 37°C in a humidified 5% CO2 atmosphere. Fibroblasts were subcultured using routine methods and were used at passages 3 to 6. Oligo-cDNA microarray hybridization was performed according to the National Cancer Institute in-house protocol, as detailed previously (Yamaguchi et al., 2008). Briefly, total RNAs were prepared from cultured fibroblasts using an RNeasy mini kit (Qiagen, Valencia, CA). The quality (purity and integrity) and quantity of each total RNA preparation was measured using a Nanodrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE). A universal human reference RNA (Stratagene, La Jolla, CA) was used as a control. cDNA samples generated from RNA samples and purified before the coupling reaction were labeled with Cy3 (for fibroblast samples) or Cy5 (for reference RNA) mono-reactive dyes (GE Healthcare, Piscataway, NJ), and were hybridized simultaneously on an oligo-DNA chip (Hs-OperonV3.0-v1p24, p27, p31) overnight at 42°C. Two fluorescent intensities of the oligo-DNA chip were scanned using a microarray scanner (GenePix 4000B; Axon Instruments Inc., Molecular Devices Corp., Sunnyvale, CA). Differential gene expression was profiled using Genepix Pro 5.0 software and was analyzed by Miltenyi Biotec (Bergisch Gladbach, Germany).

2010-08-31 | E-GEOD-22022 | biostudies-arrayexpress

Stump project -- mRNA fibroblasts; Volar versus non-volar acral skin gene expression

Project description:We were interested in defining the gene signature of volar skin. Dermal fibroblasts were expanded from punch biopsies of skin. We collected RNA from fibroblasts of palms, backs of hand, soles and backs of feet and submitted for Affymetrix Exon arrays. 3 replicates of each site from distinct human donors were included; because of 1 failed sample(Palm_rep2), total of 11 samples analyzed

2013-07-03 | E-GEOD-39266 | biostudies-arrayexpress

Stump project-- mRNA whole tissue; Volar versus non-volar acral skin gene expression

Project description:We were interesed in defining the gene signautre of volar skin. Whole skin punch biopsies of palms, backs of hand, soles and backs of feet were submitted for Affymetrix Exon arrays. 4 replicates of each site from distinct human donors were included; total of 16 samples analyzed.

2013-07-01 | E-GEOD-34756 | biostudies-arrayexpress

Stump project -- mRNA epidermis; Volar versus non-volar acral skin gene expression

Project description:We were interested in defining the gene signature of volar skin. Punch biopsies of skin were split into epidermis and dermis after dispase treatment. We collected RNA from epidermis of palms, backs of hand, soles and backs of feet and submitted for Affymetrix Exon arrays. 3 replicates of each site from distinct human donors were included; total of 12 samples analyzed

2013-07-03 | E-GEOD-39267 | biostudies-arrayexpress

Stump project-- mRNA dermis; Volar versus non-volar acral skin gene expression

Project description:We were interested in defining the gene signature of volar skin. Punch biopsies of skin were split into epidermis and dermis after dispase treatment. We collected RNA from dermis of palms, backs of hand, soles and backs of feet and submitted for Affymetrix Exon arrays. 3 replicates of each site from distinct human donors were included; total of 12 samples analyzed

2013-07-03 | E-GEOD-39743 | biostudies-arrayexpress

Genome-wide reduction in chromatin accessibility and unique transcription factor footprints in endothelial cells and fibroblasts in scleroderma skin

Project description:We assessed genome-wide chromatin accessibility and transcription factor footprinting in endothelial cells and fibroblasts isolated from skin biopsies from healthy subjects and diffuse cutaneous scleroderma patients.

2022-09-02 | GSE163199 | GEO

Methylation studies in naked mole rat. Part 1

Project description:An Infinium microarray platform (GPL28271, HorvathMammalMethylChip40) was used to generate DNA methylation data from many tissues of the naked mole rat We generated DNA methylation data from n=289 tissues. All tissues except for skin biopsies and blood were obtained from frozen tissue collection that were euthanized for other studies. Skin biopsies (2 mm punch) were collected from the backs of the animals under local anesthesia. Blood samples were collected from the tails. The n=3 induced pluripotent stem cells from NMR were generated as described in (Tan et al 2017., PMID: 29107597). As control set for the iPS study, we used n=3 fibroblasts samples. Genomic DNA was extracted using Qiagen DNeasy Blood and Tissue kit and quantified using Nanodrop and Qubit.als

2021-10-18 | GSE174777 | GEO

Microarray-based identification of age-dependent differences in gene expression of human dermal fibroblasts

Project description:Gene expression approach to differences in stress response between human skin fibroblasts derived from chronologically young (~20 years) and old (~90 years) subjects. Expression of CDKN2A was most different between fibroblasts from young and old subjects and Human skin fibroblasts were isolated from skin biopsies of 6 young subjects and 6 old subjects and were treated for 3 hours and 3 days with 0.6 uM rotenone. Also there were control samples, not treated but grown for 3 days, making a total of 36 samples. No experimental replicates were included. The expression of CDKN2A was quantified in the same RNA samples by real-time PCR.

2011-04-01 | E-GEOD-28300 | biostudies-arrayexpress

High-throughput sequencing of small RNAs from Xenopus tropicalis

Project description:High-throughput sequencing of small RNAs from Xenopus tropicalis (adult liver, adult skin, oocytes stage I, II, III, IV, V, VI). total RNA, ~18-42 nt RNAs isolated using PAGE, ligation to adapters requires 5' monophosphate and 3' OH Illumina/Solexa sequencing of adult liver, adult skin, oocytes stage I, II, III, IV, V, VI

2009-07-09 | E-GEOD-14952 | biostudies-arrayexpress

Breast Cancer Gene Expression Data from Hamburg Series

Project description:Gene expression profiling of surgical biopsies from 74 breast cancer patients of different subtypes from Hamburg dataset.

2014-04-17 | GSE46184 | GEO