Transcriptomics

Dataset Information

0

Transcriptome profiling of EPB41L5 WT and KO podocytes


ABSTRACT: We generated EPB41L5 WT and KO podocytes to model human glomerular kidney disease in vitro. Immortalized human podocytes were genome edited applying the CRISPR/Cas9 technique. Two monoclonal cell lines were generated for WT and KO genotypes (WT-1, WT-2, KO-1 & KO-2). Transcriptome profiling (RNA-Sequencing) and differential gene expression analysis was performed in duplicate for each cell line. EPB41L5 KO podocytes exhibit transcriptional changes compared to WT cells. Analyzed cell were previously described: Schell C, Rogg M, Suhm M, et al. The FERM protein EPB41L5 regulates actomyosin contractility and focal adhesion formation to maintain the kidney filtration barrier. Proc Natl Acad Sci U S A. 2017;114(23):E4621-E4630. doi:10.1073/pnas.1617004114

ORGANISM(S): Homo sapiens

PROVIDER: GSE220299 | GEO | 2023/07/03

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2022-08-20 | GSE193099 | GEO
2020-03-19 | PXD016851 | Pride
2022-04-07 | GSE189703 | GEO
2022-04-07 | GSE189709 | GEO
2024-02-08 | PXD041202 | Pride
2024-02-08 | PXD045828 | Pride
2019-01-15 | GSE116471 | GEO
2020-05-18 | PXD019251 | Pride
| PRJNA784252 | ENA
2015-11-24 | E-GEOD-66969 | biostudies-arrayexpress