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Transcriptome analysis of targeted RNA-knockdown using CRISPR-Csm in HEK293T cells

ABSTRACT: To investigate the efficacy of CRISPR-Csm complexes for RNA- knockdown in eukaryotes, we quantified transcript abundance in HEK293T cells after targeting several nuclear or cytoplasmic RNAs. RNA-seq demonstrates efficient and specific knockdown of CRISPR-Csm compared to Cas13 and shRNA knockdown.

ORGANISM(S): Homo sapiens

PROVIDER: GSE220741 | GEO | 2022/12/12

REPOSITORIES: GEO

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