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The AP-1 transcription factor complex is required for avian neural crest cell specification

ABSTRACT: During embryonic development, cellular identity relies upon the complex interplay between signaling systems, transcription factors, and cis-regulatory elements. This is epitomized in the neural crest, whose formation is governed by a comprehensive gene regulatory network (GRN) jumpstarted by several signaling systems including Wnt, BMP, and FGF. Although there is substantial knowledge regarding the nuclear effectors and downstream target genes of Wnt and BMP signaling in neural crest formation; the effectors of FGF signaling remain unclear. Recent work from our group has demonstrated a decisive role for the FGF/MAPK/AP-1 axis in modulating the human neural crest epigenome. Nevertheless, whether this regulation remains conserved across vertebrate species remains unknown. To address this, we examined the activity and requirement of the AP-1 transcription factor complex in avian neural crest cell formation. Functional characterization of AP-1 demonstrated it is a major regulator of avian neural crest identity, directly regulating the expression of several GRN components. Furthermore, we demonstrate that AP-1 serves as a nuclear effector of FGF signaling in avian neural crest cells, consistent with our previous results observed in human neural crest. Together, these results demonstrate the conserved activity of AP-1 required for neural crest specification and can facilitate its incorporation into the GRN.

ORGANISM(S): Gallus gallus

PROVIDER: GSE221031 | GEO | 2024/12/31

REPOSITORIES: GEO

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