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Repairing growth of a genome-reduced strain using adaptive laboratory evolution and targeted gene knock-in

ABSTRACT: We profiled growth of an evolved version of the genome reduced E. coli strain DGF-298 in various growth conditions. We used RNA-seq to compare this strain's growth to the parent wild-type strain. We also profiled the transcriptional effect of adding in a removed gene predicted by models to have a positive impact on growth (aldA).

ORGANISM(S): Escherichia coli

PROVIDER: GSE221155 | GEO | 2022/12/20

REPOSITORIES: GEO

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