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Distinctive Interactomes of RNA polymerase II phosphorylation during different stages of transcription

ABSTRACT: To investigate the whole-transcriptome effects of Calcium Homeostasis Endoplasmic Reticulum Protein (CHERP)/SCAF6 which specifically binds to the Ser2 of C-terminal domain of RNA Polymerase II, we established HEK293 cell line in which SCAF6 gene was knocked down by shRNA. We then performed gene expression and alternative splicing profiling using data obtained from RNA-seq of 2 cell lines (HEK293 Control vs HEK293 SCAF6 KD).

ORGANISM(S): Homo sapiens

PROVIDER: GSE221328 | GEO | 2023/08/17

REPOSITORIES: GEO

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Distinctive Interactomes of RNA polymerase II phosphorylation during different stages of transcription

Project description:We have discovered distinctive protein interactomes for Ser2 and Ser5 phosphorylation of the C-terminal domain (CTD) of RNA Pol II. Calcium Homeostasis Endoplasmic reticulum (CHERP) was identified in our pulldown to directly interact with Ser2 phosphomarks on the CTD of Pol II. As Pol II is a chromatin-associated protein, we sought to identify genomic regions that CHERP, a Pol II binding protein, occupy through ChIP-seq analysis.

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Distinctive Interactomes of RNA polymerase II phosphorylation during different stages of transcription

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