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Single cell RNA sequencing profiles of human iPSC-derived AT1-like cells in 3D cultures [SPC2B2_LDCI]

ABSTRACT: Comparison of differentiation of human iPSC derived alveolar epitheilal type 2 cells (iAT2s) into AT1-like cells (iAT1s) using a defined "LDCI" medium vs. lentiviral forced over-expression of activated nuclear YAP (YAP5SA)

ORGANISM(S): Homo sapiens

PROVIDER: GSE221343 | GEO | 2023/08/08

REPOSITORIES: GEO

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Nuclear YAP overexpression drives the human alveolar type 1 cell (AT1) cprogram in a cell autonomous manner in lentiviral transduced iPSC-derived alveolar type 2 cells (iAT2s)

Project description:SPC2B2 iAT2s were transduced with either control WT YAP or constitutively active nuclear YAP (YAP5SA) lentivirus in a single cell suspension and were then grown in iAT2 media (CK + DCI) in 3D matrigel for 7 days.

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