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Transcriptome analysis of the over-expression of OsMYB14, an R2R3-MYB Transcription Factor in Rice

ABSTRACT: The profiling was conducted with the Rice 3'-Tiling 135k Microarray designed from 31,439 genes deposited at IRGSP, RAP2 database (http://rapdb.lab.nig.ac.jp). In this research, we have searched for genes responsible for over-expression of OsMYB14, an R2R3-MYB transcription factor in rice, Oryza sativa cv IlMi

ORGANISM(S): Oryza sativa

PROVIDER: GSE221391 | GEO | 2022/12/20

REPOSITORIES: GEO

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Analysis of representative organ specific genes and promoters of rice using 3’ ORF oriented long oligomer microarray

Project description:The profiling was conducted with the Rice 3'-Tiling Microarray designed from 27,448 genes deposited at IRGSP, RAP1 database (http://rapdb.lab.nig.ac.jp). In this research, an array of 27,448 rice genes was used to elucidate the transcriptome of 7 tissues or organs of Oryza sativa L. cv. Dongjin including calli, regenerating calli, germinating seeds, leaves, roots, and flowers (before and after pollination) using a rice 3′ ORF tilling microarray. The ratio of standard deviation to the mean of microarray intensities was used to distinguish between organ-specific and constitutively expressed genes. Accordingly, the genes are classified into highly variable, variable, and constitutive groups. To isolate the organ-specific promoters, several genes were selected and validated in planta using reporter gene analysis. We found that the Os01g0702500, Os11g0211800, and Os01g0257300 promoters were active in the calli, germinating seeds, and roots, respectively. The Os08g0135500 promoter was shown to drive transgene expression in various organs of the mature flowers, such as the anther, lemma, and palea, whereas the Os03g0369100 promoter was only active in the anther. Lastly, the Os09g0553100 promoter induced high levels of reporter gene expression in all organs. The gene expression data from representative organs could put a frame work for large dataset collections and then subsequent profiling by subdivision of organ/tissues might be more efficient to find appropriate promoters.

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Transcriptome analysis of the over-expression of OsMYB14, an R2R3-MYB Transcription Factor in Rice

Project description:Transcriptome analysis of the over-expression of OsMYB14, an R2R3-MYB Transcription Factor in Rice

| PRJNA914235 | ENA

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