Periplasmic stress contributes to a tradeoff between protein secretion and cell growth in E. Coli Nissle
Ontology highlight
ABSTRACT: To investigate changes in E. coli Nissle 1917 gene expression when engineered to secrete a large amount of protein via the curli (Type VIII) secretion system.
Project description:To determine whether calprotectin can elicit any transcriptional response in the probiotic E. coli Nissle 1917(EcN), EcN was treated with 200 ug/g of calprotectin in log phase.
Project description:RNA sequencing of Escherichia coli Nissle 1917 before and after HOCl treatment was perfomed to identify pathways that may be important in responding to oxidative stress caused by reachive chlorine species (RCS).
Project description:Confluent Caco-2 cells cultured over 6 hours non-treated with E.coli Nissle 1917; (GSM40938 and GSM40941). Confluent Caco-2 cells cocultured over 6 hours with E.coli Nissle 1917 (GSM40881 and GSM40937).
Project description:We performed comparative transcriptomic profiling of E. coli Nissle 1917 (EcN) to determine the effect of microgravity (MG) on cell growth and metabolism.