Methylation profiling

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Targeted bisulfite sequencing to profile age-associated DNA methylation changes in Xenopus frogs


ABSTRACT: Here we present clear patterns of age-associated changes in DNA methylation in the aquatic vertebrate species Xenopus tropicalis. We selected approximately 3500 sites highly correlated with age from WGBS data from 9 frogs of 3 different age groups (young, approx. 1 year old; mature, approx. 5 years old; old, approx. 9 years old - GSE221656) and designed probes for the development of cost effective targeted bisulfite sequencing (TBSeq) assay. Here, we present the TBSeq assay in sixteen different frogs as a pilot experiment to validate our previous findings.

ORGANISM(S): Xenopus tropicalis

PROVIDER: GSE222107 | GEO | 2023/04/28

REPOSITORIES: GEO

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