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Gene expression alterations in the cell lines Jurkat, THP1, and KHYG-1 upon treatment with glycodelin from non-small-cell lung cancer cell line supernatant

ABSTRACT: The function of glycodelin A in the endometrium, during the menstrual cycle, and before or during pregnancy is well characterized. Numerous studies have investigated the highly pleiotropic effects and the modulation of different leukocytes upon glycodelin interaction. In NSCLC, the function of glycodelin is not known, yet. While it was shown to be highly expressed in lung tumor tissue compared to normal lung, the functionality and possible immunomodulating characteristics remain to be described.

ORGANISM(S): Homo sapiens

PROVIDER: GSE222124 | GEO | 2023/01/09

REPOSITORIES: GEO

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Project description:Glycodelin is a major glycoprotein expressed in reproductive tissues, like secretory and decidualized endometrium. It has several reproduction related functions that are dependent on specific glycosylation, but it has also been found to drive differentiation of endometrial carcinoma cells toward a less malignant phenotype. Here we aimed to elucidate whether the glycosylation and function of glycodelin is altered in endometrial carcinoma as compared with a normal endometrium. We carried out glycan structure analysis of glycodelin expressed in HEC-1B human endometrial carcinoma cells (HEC-1B Gd) by mass spectrometry glycomics strategies. Glycans of HEC-1B Gd were found to comprise a typical mixture of high-mannose, hybrid, and complex-type N-glycans, often containing undecorated LacNAc (Gal?1-4GlcNAc) antennae. However, several differences, as compared with previously reported glycan structures of normal human decidualized endometrium-derived glycodelin isoform, glycodelin-A (GdA), were also found. These included a lower level of sialylation and more abundant poly-LacNAc antennae, some of which are fucosylated. This allowed us to select lectins that showed different binding to these classes of glycodelin. Despite the differences in glycosylation between HEC-1B Gd and GdA, both showed similar inhibitory activity on trophoblast cell invasion and peripheral blood mononuclear cell proliferation. For the detection of cancer associated glycodelin, we established a novel in situ proximity-ligation based histochemical staining method using a specific glycodelin antibody and UEAI lectin. We found that the UEAI reactive glycodelin was abundant in endometrial carcinoma, but virtually absent in normal endometrial tissue even when glycodelin was strongly expressed. In conclusion, we established a histochemical staining method for the detection of endometrial carcinoma-associated glycodelin and showed that this specific glycodelin is exclusively expressed in cancer, not in normal endometrium. Similar methods can be used for studies of other glycoproteins.

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Gene expression alterations in the cell lines Jurkat, THP1, and KHYG-1 upon treatment with glycodelin from non-small-cell lung cancer cell line supernatant

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