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Transcriptomic and Metabolomic Changes Reveals the Immunomodulatory Function of Casein Phosphopeptide-Selenium Chelate in Beagle Dogs

ABSTRACT: Casein phosphopeptide-selenium complex (CPP-Se), an organic chelate of casein phosphopeptide and selenium, has been shown to stimulate canine lymphocytes proliferation and cytokines production in our previous research, but its effect on the gene expression of peripheral blood is unclear. To further investigate the potential mechanism associated with CPP-Se in the process of exerting immunomodulatory function in dogs, our current study used high-throughput sequencing technology to explore the effects of feeding CPP-Se for 30 days on the blood transcriptome of dogs. Compared with control group, a total of 341 differentially expressed genes (DEGs) were identified in CPP-Se group, of which 110 were up-regulated and 231 were down-regulated. Gene ontology (GO) analysis revealed that DEGs were enriched in various biological processes and molecular functions, such as ion transport, transmembrane signaling receptor activity, molecular transducer activity and cytokine activity. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis found that DEGs were mainly implicated in multiple immune-related signaling pathways. Immune-related DEGs were screened by the Immport database and the hub genes of all DEGs or DEGs related to cytokine-cytokine receptor interaction pathway and T cell receptor signaling pathway were identified by Cytoscape. Finally, the reliability of RNA-Seq data was verified by RT-qPCR. Our findings indicated that CPP-Se can change the blood gene expression profile of dogs and the results will serve as a scientific reference for the future development of functional foods to improve pet immunity, as well as a theoretical basis for monitoring and evaluating the immune status of dogs.

ORGANISM(S): Canis lupus familiaris

PROVIDER: GSE222161 | GEO | 2023/05/31

REPOSITORIES: GEO

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Project description:Interventions: Participants will take 6 brazil nuts a day for four weeks. This four week intervention will be preceded by a 4 week run-in / wash out phase participants will be advised to exclude any selenium rich foods or green tea during the study This Pilot study will confirm the amount of Brazil Nuts we will use in the study comparing with green tea. This follow up study (Effects of combining Selenium (Se) and green tea on biomarkers for colorectal cancer prevention in human subjects). will be registered with ANZCTR in the near future’ At the initial interview dietary advice will be given. This will give an understanding of the nature of the volunteers diet and the likely influence of Se to the diet and suitability to become involved in the study (if intolerant to nuts volunteers will not participate in the study) We will ask participants not to consume, whenever possible, throughout the study:- Any selenium supplement Any selenium enriched foods, such as nuts, sea food (such as tuna or octopus) The Brazil nut is a large nut that comes from the castanheiro de para tree in Brazil’s rainforests. Each serving of about six to eight nuts contains 4 g of protein and 7 grams each of monosaturated and polysaturated fat. Brazil nuts (Berholletia excelas, family Lecythidaceae) are the richest known food source of selenium, with mean concentrations reported in the literature between 8-83 micrograms Se/g 6 Brazil nuts daily provides 48 micrograms Se/day - 53 micrograms Se/day Primary outcome(s): If supplementation of 6 Brazil nuts/day increases plasma Selenium (Se) levels A blood sample (5mls of whole blood taken on 2 occasions) will be collected at baseline and after intervention, testing for plasma Selenium levels[Blood samples collected at commencement of intervention (week 0) and at end of intervention (wk 4)] Study Design: Purpose: Prevention; Allocation: Non-randomised trial; Masking: Open (masking not used);Assignment: Single group;Type of endpoint: Safety

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Transcriptomic and Metabolomic Changes Reveals the Immunomodulatory Function of Casein Phosphopeptide-Selenium Chelate in Beagle Dogs

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