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Examining the Effect of Notocactus ottonis Cold Vacuum Isolated Cell Extract on Hair Growth in C57BL/6 Mice using a Combination of Physiology and OMICS analyses

ABSTRACT: This study focuses on the hair-growing effects of a cell extract (CE) obtained from the cactus Notocactus ottonis by a cold vacuum extraction protocol, with the aim of investigating its hair-growing effects and relevant mechanisms and potential factors therein. In an experiment using male C57BL/6 mice, a vehicle control group (VC: propylene glycol : ethanol : water), a positive control group (MXD : 3% minoxidil), and an experimental group (N-CE: 10% N. ottonis CE) were applied topically to the backs of mice. Results showed that 3% MXD and 10% N-CE were more effective in promoting hair growth than the VC. Furthermore, an increase in the number of hair follicles was observed with 10% N-CE in hematoxylin-eosin-stained skin tissue.

ORGANISM(S): Mus musculus

PROVIDER: GSE222210 | GEO | 2023/01/06

REPOSITORIES: GEO

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Examining the Effect of Notocactus ottonis Cold Vacuum Isolated Cell Extract on Hair Growth

Project description:The biological and psychological importance of hair is recognized worldwide. Molecules that can promote the activation of hair follicle stem cells and initiation of the growth phase have been the subject of much research. Clarifying how hair regeneration is regulated may help to provide hair loss treatments, including cosmetic and even psychological interventions. This study focuses on the hair-growing effects of a cell extract (CE) obtained from the cactus Notocactus ottonis by a cold vacuum extraction protocol, with the aim of investigating its hair-growing effects and relevant mechanisms and potential factors therein. In an experiment using female C57BL/6 mice, a vehicle control group (VC: propylene glycol : ethanol : water), a positive control group (MXD : 3% minoxidil), and an experimental group (N-CE: 10% Notocactus ottonis CE) were applied topically to the backs of mice. Results showed that 3% MXD and 10% N-CE were more effective in promoting hair growth than the VC. Furthermore, an increase in the number of hair follicles was observed with 10% N-CE CE in hematoxylin-eosin-stained skin tissue. We also examined the metabolite composition of the N-CE. Additionally, the whole genome DNA microarray and total protein profiling was carried out on the skin samples to suggest molecular factors underlying the hair-promoting effects of N-CE. This study raises the possibility of using the newly described Notocactus ottonis cell extract as a hair-growth promoting agent.

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DNA Microarray and Bioinformatic Analysis Reveals the Potential of Whale Oil in Enhancing Hair Growth in C57BL/6 Mice

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Project description:Children ages 0-10 years old with CF were recruited from 2012-2015 at the outpatient CF clinic, and classified according to age (infants <1 year old, vs. children 1-10 years old). The diagnosis of CF was defined as two disease-causing mutations or a sweat chloride test ≥ 60 mmol/L. Hair and blood samples were collected from each subject. Hair nicotine concentrations were determined and considered as the primary objective measure of SHSe. Hair nicotine provides a long-term measure of SHSe as nicotine is integrated into the growing hair shaft over multiple months. (15) For each subject, 30-40 shafts of hair of approximately 2-3 cm in length were cut at the hair root from the occipital skull. Hair samples were refrigerated at 4° for storage, washed before analyses to remove ambient nicotine (15) and batch-tested at a contract research facility (Environmental Health Sciences, Johns Hopkins School of Public Health). Samples were processed by reverse-phase high-performance liquid chromatography with electrochemical detection as described. (15) Hair nicotine concentrations were expressed as ng/mg of hair and the assay limit of detection was 0.087 ng/mg.

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Project description:In the presence of environmental change, natural selection can shape the transcriptome. Under a scenario of environmental change, genotypes that are better able to modulate gene expression to maximize fitness will tend to be favored. Therefore, it is important to examine gene expression at the population level in order to distinguish random or neutral gene expression variation from the pattern produced by natural selection. This study investigates the natural variation in transcriptional response to a cactus host shift utilizing the mainland Sonora population of Drosophila mojavensis. Drosophila mojavensis is a cactophilic species composed of four cactus host populations endemic to the deserts of North America. Overall, the change in cactus host was associated with a significant reduction in larval viability, as well as the differential expression of 21% of the genome (3,109 genes). Among the genes identified were a set of genes previously known to be involved in xenobiotic metabolism, as well as genes involved in cellular energy production, oxidoreductase/carbohydrate metabolism, structural components and mRNA binding. Interestingly, of the 3,109 genes whose expression was affected by host use, there was a significant overrepresentation of genes that lacked an orthologous call to the D. melanogaster genome, suggesting the possibility of an accelerated rate of evolution in these genes. Of the genes with a significant cactus effect, the majority, 2,264 genes, did not exhibit a significant cactus-by-line interaction. This population level approach facilitated the identification of genes involved in past cactus host shifts. Dataset from Population transcriptomics of cactus host shifts in Drosophila mojavensis, Matzkin, LM. Molecular Ecology.

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