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Next Generation Sequencing Facilitates Transcriptomes Quantitative Analysis of N2A cells transfected with the plasmids respectively encoding RfxCas13d/dRfxCas13d and crRNA.

ABSTRACT: To determine what effect the collateral activity of RfxCas13d has on N2acells. The plasmids encoding RfxCas13d/dRfxCas13d and crRNA were transfected into N2a cells. After 48 hours of transfection, GFP-positive cells were collected using FCAS and extracted for total RNA. Then, we analyzed the transcriptome of each group with RNA-seq and analyzed the differential genes among the groups.

ORGANISM(S): Mus musculus

PROVIDER: GSE222461 | GEO | 2023/01/14

REPOSITORIES: GEO

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