Transcriptomics

Dataset Information

0

Bulk RNA-seq of CRISPRi ADGRG6-kd iAT2 following cigarette smoke exposure


ABSTRACT: We performed bulk transcriptomic profiling of induced human pluripotent stem cells (iPSCs)-derived type 2 alveolar epithelial cells (iAT2). iPSCs stably expressed CRISPRi (dCas9-KRAB) under the control of doxycyline. iAT2s were transduced with a lentivirus expressing gRNA targeting the transcriptional start site of ADGRG6. Cells were treated with or without doxycyline to intiate CRISPRi-knockdown. Cells were plated at an air-liquid interface, then subsequently exposed to air or 5% cigarette smoke using a VitroCell smoke robot. Cells were harvested for bulk RNA sequencing 8 hours post cigarette smoke exposure

ORGANISM(S): Homo sapiens

PROVIDER: GSE223077 | GEO | 2023/09/20

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2023-09-20 | GSE223078 | GEO
2022-05-26 | GSE189068 | GEO
| PRJNA924805 | ENA
| PRJNA924804 | ENA
| PRJNA781443 | ENA
2022-09-30 | GSE205093 | GEO
2022-01-03 | GSE186976 | GEO
2005-02-23 | GSE2302 | GEO
2012-07-22 | E-GEOD-33512 | biostudies-arrayexpress
2022-04-30 | GSE184142 | GEO