Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

T7-SAGE transcriptome of cleft versus end bud epithelial cells of embryonic mouse salivary glands

ABSTRACT: Mus musculus embryonic submandibular salivary glands from embryonic day 12.5 embryos were laser microdissected to isolate epithelial cells from regions adjacent to forming clefts or from the peripheral end bud region. The isolated region-specific samples were then subjected to T7-SAGE transcriptome analysis.

ORGANISM(S): Mus musculus

PROVIDER: GSE22374 | GEO | 2010/06/17

SECONDARY ACCESSION(S): PRJNA127655

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

T7-SAGE transcriptome of cleft versus end bud epithelial cells of embryonic mouse salivary glands

Project description:Mus musculus embryonic submandibular salivary glands from embryonic day 12.5 embryos were laser microdissected to isolate epithelial cells from regions adjacent to forming clefts or from the peripheral end bud region. The isolated region-specific samples were then subjected to T7-SAGE transcriptome analysis. Toluidine blue-stained cryostat sections of embryonic day 12.5 submandibular salivary glands were used for laser microdissection using an LMD system (Leica) with a pulsed UV laser on an upright automated microscope. A total of approximately 500 cells from a region immediately adjacent to a forming cleft or from a peripheral end bud region were excised by the laser and transferred to caps of 0.5 ml PCR tubes. SAGE libraries were constructed from the microdissected cleft and bud epithelia cells. SAGE tags were identified using the National Center for Biotechnology Information (NCBI) tag map set for UniGene.

2010-06-16 | E-GEOD-22374 | biostudies-arrayexpress

Escherichia phage T7 strain:T7

Project description:Escherichia phage T7 strain:T7 Genome sequencing and assembly

| PRJNA733815 | ENA

Paenibacillus massiliensis T7 strain:T7

Project description:Paenibacillus massiliensis T7 strain:T7 Genome sequencing and assembly

| PRJNA202952 | ENA

Rhodococcus sp. T7 strain:T7

Project description:Rhodococcus sp. T7 strain:T7 Genome sequencing and assembly

| PRJNA598817 | ENA

Plasmodium falciparum MSRP6-C-terminal domain (cd) interactome vs Maurer’s Cleft proteome and host cytosolic proteome using quantitative BioID

Project description:Interaction partners to a small part of the Maurer's clefts protein MSRP6 termed 'cd' were identified using BioID. The cd part recruits MSRP6 to the Maurer's clefts. To find the interactors of cd in isolation from MSRP6, this region was appended together with BirA* (for BioID) to a truncated version of REX3 to traffic it to the host cell (construct REX3trunc-3cd-BirA*-GFP). This construct is found at the Maurer's clefts by virtue of the cd domain. To obtain a BioID proteome specific for cd, two control constructs were generated. First, REX3trunc-BirA*-GFP without cd (freely soluble in the host cell providing a host cytosolic proteome) and STEVOR1-260-BirA*-GFP (as a control for general Maurer's clefts proteins proving a Maurer’s Cleft proteome). Quantitative mass spectrometry was then used to identify candidates for cd-specific MSRP6 interaction partners compared to general Maurer's clefts proteins and exported proteins not localizing at the Maurer's clefts.

2022-03-21 | PXD008208 | Pride

Saccharomyces cerevisiae T7 strain:T7

Project description:Saccharomyces cerevisiae T7 genome sequencing

| PRJNA60387 | ENA

Escherichia phage T7

Project description:Enterobacteria phage T7 overview

| PRJNA69579 | ENA

Teseptimavirus T7

Project description:Teseptimavirus T7 Genome sequencing and assembly

| PRJNA481178 | ENA

CNV analysis of 869 individuals from the Philippines with cleft lip and/or cleft palate

Project description:Cleft lip with or without cleft palate (CL/P) is a common birth defect with a complex, heterogeneous etiology. It is well-established that both common and rare sequence variants contribute to the formation of CL/P, however, the contribution of copy number variants (CNVs) to cleft formation remains relatively understudied. To fill this knowledge gap, we conducted a large-scale comparative analysis of genome-wide CNV profiles of 869 individuals from the Philippines and 233 individuals of European ancestry with CL/P with three primary goals: first, to evaluate whether differences in CNV number, amount of genomic content, or amount of coding genomic content existed within clefting subtypes; second, to assess whether CNVs in our cohort overlapped with known Mendelian clefting loci; and third, to identify unestablished Mendelian clefting genes. Significant differences in CNVs across cleft types or in individuals with non-syndromic versus syndromic clefts were not observed, however, several CNVs in our cohort overlapped with known syndromic and non-syndromic Mendelian clefting loci. Moreover, employing a filtering strategy relying on population genetics data that rare variants are on the whole more deleterious than common variants, we identify several CNV-associated gene losses likely driving non-syndromic clefting phenotypes. By prioritizing genes deleted at a rare frequency across multiple individuals with clefts yet enriched in our case cohort compared to controls, we identify COBLL1, RIC1, and ARHGEF38 as clefting genes. CRISPR/Cas9 mutagenesis of these genes in Xenopus laevis and Danio rerio yielded craniofacial dysmorphologies, including clefts analogous to those seen in human clefting disorders.

2022-12-01 | GSE212166 | GEO

Escherichia phage T7

Project description:Escherichia phage T7 Genome sequencing

| PRJNA205854 | ENA