Transcriptomics

Dataset Information

0

Single-cell RNA sequencing reveals cellular and molecular heterogeneity in fibrocartilaginous enthesis formation


ABSTRACT: The attachment site of the rotator cuff (RC) is a classic fibrocartilaginous enthesis, which is the junction between bone and tendon with typical characteristics of a fibrocartilage transition zone. Enthesis development has historically been studied with lineage tracing of individual genes selected a priori, which does not allow for the determination of single-cell landscapes yielding mature cell types and tissues. Here, in together with open source GSE182997 datasets (3 sample) provided by Fang et al, we applied Single-cell RNA sequencing (scRNA-seq) to delineate the comprehensive postnatal RC enthesis growth and the temporal atlas from as early as postnatal day 1 up to postnatal week 8. And we furtherly performed single cell spatial transcriptomic sequencing on postnatal day 1 mice enthesis, in order to deconvoluted bone-tendon junction (BTJ) chondrocytes onto spatial spots. In summary, we deciphered the cellular heterogeneity and the molecular dynamics during fibrocartilage differentiation. Combined with current spatial transcriptomic data, our results provide a transcriptional resource that will support future investigations of enthesis development at the mechanistic level and may shed light on the strategies for enhanced RC healing outcomes.

ORGANISM(S): Mus musculus

PROVIDER: GSE223751 | GEO | 2023/09/20

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2022-12-01 | GSE182997 | GEO
2021-06-11 | GSE154525 | GEO
| PRJNA719822 | ENA
2024-07-01 | GSE256128 | GEO
2023-05-31 | GSE226742 | GEO
2024-06-10 | GSE268244 | GEO
2021-06-11 | GSE154397 | GEO
2021-11-11 | GSE188540 | GEO
2023-03-02 | E-MTAB-12529 | biostudies-arrayexpress
2019-04-24 | GSE130191 | GEO