Transcriptomics

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Real-time quantitative PCR analysis of human induced T regulatory cells (Tregs) for metabolic enzymes


ABSTRACT: Human naïve CD4 T cells were purified from healthy volunteers' blood and were differentiated into induced Tregs in vitro by culturing for 5 days in the presence of anti-CD3 and CD28 antibodies (2 µg/ml each), IL-2 (100 units/ml) and TGF-β1 (5 ng/ml) for 5 days, then in presence of anti-CD3 and CD28 antibodies for 2 days. Human iTregs were harvested on day 7 post differentiation, then treated with either vehicle or IL-21 (100 ng/ml) in serum-free RPMI media for 18 hours.

ORGANISM(S): Homo sapiens

PROVIDER: GSE224033 | GEO | 2023/04/30

REPOSITORIES: GEO

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