Transcriptomics

Dataset Information

0

Identification and characterization of two novel primate-specific histone H3 variants H3.X and H3.Y


ABSTRACT: Nucleosomal incorporation of specialized histone variants is an important mechanism to generate different functional chromatin states. Here we report the identification and characterization of two novel primate-specific histone H3 variants, H3.X and H3.Y. Their mRNAs are found in certain human cell lines, in addition to several normal and malignant human tissues. In keeping with their primate-specificity, H3.X and H3.Y are detected in different brain regions. Transgenic H3.X and H3.Y proteins are stably incorporated into chromatin in a similar fashion to the known H3 variants. Importantly, we demonstrate biochemically and by mass spectrometry that endogenous posttranslationally modified H3.Y protein exists in vivo, and that stress-stimuli, such as starvation and cellular density, increase the abundance of H3.Y-expressing cells. Global transcriptome analysis revealed that knock-down of H3.Y affects cell growth and leads to changes in the expression of many genes involved in cell cycle control. Thus, H3.Y is a novel histone variant involved in the regulation of cellular responses to outside stimuli.

ORGANISM(S): Homo sapiens

PROVIDER: GSE22415 | GEO | 2010/09/17

SECONDARY ACCESSION(S): PRJNA128575

REPOSITORIES: GEO

Shared Molecules

Only show the datasets with similarity scores above: 0.5
     

Similar Datasets

2010-09-17 | E-GEOD-22415 | biostudies-arrayexpress
2009-02-01 | E-TABM-623 | biostudies-arrayexpress
2015-07-20 | GSE63890 | GEO
2017-03-13 | GSE94034 | GEO
2017-07-28 | PXD005300 | Pride
| PRJNA128575 | ENA
| PRJNA489180 | ENA
2023-07-27 | MSV000092535 | MassIVE
| PRJNA489182 | ENA
| PRJNA489184 | ENA