Transcriptomics

Dataset Information

0

Expression data for naïve IL-2 and IL-12 primed Pmel-1 CD8+ T-cells


ABSTRACT: The expansion, trafficking and functional effectiveness of adoptively transferred CD8+ T-cells play a critical role in mediating effective anti-tumor immunity. However, the mechanisms which program the highly proliferative and functional state of CD8+ T-cells are not completely understood. We hypothesized that IL-12, a cytokine commonly induced by TLR activation, could enhance T-cell priming by altering responsiveness to antigen and cytokines. Priming of tumor specific CD8+ T-cells in the presence of IL-12 induced the acquisition of a 'polyfunctional' effector response and increased the generation of memory cells. Moreover, IL-12 priming also promoted high levels of the IL-2 receptor alpha-chain (CD25) and robust IL-2 mediated activation of STAT5. This sensitivity to IL-2 translated into enhanced in vivo proliferation of adoptively transferred CD8+ T-cells. Furthermore, real-time, in vivo imaging of T-cell trafficking confirmed the ability of IL-12 priming to drive in vivo proliferation. IL-12 priming enhanced the anti-tumor function of adoptively transferred cells by reducing established subcutaneous tumor burden, and significantly increasing survival in an established intracranial tumor model. Finally, IL-12 priming of human PBMCs generates tumor specific T-cells phenotypically and functionally similar to IL-12 primed Pmel-1 T-cells. These results highlight IL-12 as an important mediator of CD8+ T-cell effector function and anti-tumor immunity.

ORGANISM(S): Mus musculus

PROVIDER: GSE22443 | GEO | 2010/10/19

SECONDARY ACCESSION(S): PRJNA128523

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2010-10-19 | E-GEOD-22443 | biostudies-arrayexpress
2012-04-22 | E-GEOD-29164 | biostudies-arrayexpress
2012-04-23 | GSE29164 | GEO
2022-02-22 | PXD022909 | Pride
2013-11-21 | GSE49274 | GEO
2013-11-21 | E-GEOD-49274 | biostudies-arrayexpress
2012-07-19 | E-GEOD-39508 | biostudies-arrayexpress
2015-02-19 | E-GEOD-59454 | biostudies-arrayexpress
| PRJNA694569 | ENA
2024-01-26 | PXD044740 | Pride