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Effects of USB1 knockout on global miRNome in K562 cells

ABSTRACT: USB1 mutations lead to poikiloderma with neutropenia. We investigated the effect of inactivating USB1 on the global miRome in K562 cells

ORGANISM(S): Homo sapiens

PROVIDER: GSE224430 | GEO | 2023/02/07

REPOSITORIES: GEO

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Effects of USB1 mutation on global miRNome during hESC-derived definitive hematopoiesis

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Global 3' RACE in yeast and humans

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Transcription profiling by high throughput sequencing of MPN1 deficient human cells

Project description:Clericuzio-type poikiloderma with neutropenia (PN, Mendelian Inheritance in Man (MIM) accession 604173) is a rare genodermatosis associated to mutations in the MPN1 gene. The aim of this experiment was to investigate the the effect of MPN1 mutations on pre-mRNA transcripts maturation. We compared the transcriptome obtained from 2 lymphoblastoid immortal cell lines from PN patients with the one of a lymphoblastoid cell line established from the healthy brother of one of the patient. The trasncriptome was generated by single-end RNA-Seq.

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