Project description:We have established AR3 transgenic mouse models with targeted expression of AR3 in the prostate using the ARR2PB promoter. We have carried out gene expression profiling in AR3 transgenic prostate and wild-type prostate tissues respectively. We performed gene expression profiling in AR3 transgenic prostates to identify differentially expressed genes in the AR3Tg at the age of 4 weeks (n=3), wild-type littermates (n=3) were used as controls.
Project description:We performed expression profiling of prostates of 3 month wild-type and PTEN NULL mice and assessed the response to 3 days of castration.
Project description:Single-cell RNA-sequencing was conducted on heterogeneous human prostates FACS sorted for viability from BPH patients on the 10x Genomics platform.
Project description:Attenuation of Hedgehog (Hh) pathway activity leads to increased prostate branching during adult prostate regeneration. In order to identify genes regulated by the Hh pathway that might be involved in adult prostate branching, we performed transcriptional profiling of regenerating prostates from wild-type or Gli1-/- mutant mice, which have attenuated Hh pathway activity. 6 total samples were analyzed. 3 wild-type prostates and 3 Gli-/- mutant prostates were analyzed.
