Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptomic studies in NR4A1 overexpressing MIN6 cells and the effect of EGCG by RNA seq analysis

ABSTRACT: MIN6 cells were transfected with pLenti-NR4A1 plasmid and the expression profile of genes was studied using NGS

ORGANISM(S): Mus musculus

PROVIDER: GSE224812 | GEO | 2024/08/31

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcriptomic studies in NR4A1 overexpressing MIN6 cells and the effect of EGCG by RNA seq analysis

Project description:Transcriptomic studies in NR4A1 overexpressing MIN6 cells and the effect of EGCG by RNA seq analysis

| PRJNA932737 | ENA

RNA-seq of MIN6 cells overexpressing Hnf1a through CRISPR activation

Project description:To determine the impact of Hnf1a overexpression on gene expression in mouse beta cells, we performed RNA-seq experiments on MIN6 where Hnf1a was overexpressed using CRISPR activation (SAM system).

2022-05-01 | E-MTAB-11474 | biostudies-arrayexpress

Identification of a novel Glis3-348aa protein in MIN6 cells by LC-MS

Project description:To identify the novel protein encoded by circGlis3, total protein lysates were extracted from MIN6 cells transfected with circGlis3 plasmid or empty vector and separated by SDS-PAGE, followed by Coomassie brilliant blue staining. The differential gel bands near 35 kDa were excised and subjected to mass spectrometry.

2023-11-02 | PXD037447 | Pride

MIN6 beta cell CtBP2 ChIP-seq

Project description:CtBP2 ChIP-seq analysis was performed in a murine pancreatic beta cell line MIN6.

2023-08-08 | GSE221257 | GEO

Expression data from Min6 cells overexpressing control or SPARC adenovirus

Project description:Expression data from Min6 cells overexpressing control or SPARC adenovirus

| PRJNA649948 | ENA

RNA-seq of MIN6 cells overexpressing Hnf1a through CRISPR activation

Project description:RNA-seq of MIN6 cells overexpressing Hnf1a through CRISPR activation

| PRJEB51149 | ENA

Gene Expression of Kidney from FHH and Nr4a1 Deficient Rats

Project description:Nr4a1 deficient rats (Nr4a1-/-) were developed using the fawn hooded hypertensive rat (FHH), which provided a genetic background susceptible to kidney injury. Both groups of animals were evaluated for blood pressure, proteinuria, renal function, and whole transcriptome gene pathway changes. Gene expression profiling was performed at week 8, 16, and 24 using kidney from FHH and Nr4a1-/- rats. To identify differentially expressed gene between FHH and Nr4a1-/- two statistical methods were utilized: (1) FWER (family-wise error rate) procedure, p<0.05 and fold-change M-BM-11.2 or greater; and/or (2) Benjamani and Hochberg FDR (false discovery rate) using p<0.05, and fold-change M-BM-11.2 or greater. Two-way ANOVA using a p<0.01 or lower was performed to identify strain X time interaction effects between groups. Gene networks and functional analysis were evaluated through the use of Ingenuity Pathways Analysis . FHH and Nr4a1-/- rats were grown to 8, 16, and 24 weeks of age and kidney tissue was harvested for transcriptome analysis (n=3 replicates/ per group/ time)

2014-02-06 | E-GEOD-54714 | biostudies-arrayexpress

Microarray Analysis of MIN6 cells treated with Exendin-4 over time

Project description:The aim of the study was to investigate the effect of Exendin-4 on MIN6 cells allowing for the elucidation of the various transcriptional programs initiated by this multifunctional peptide hormone. MIN6 cells were treated with Exendin-4 for 24, 48 and 72 hours. After these time points RNA was isolated and used for hybridization (on Cy3) against matched control MIN6 cells (on Cy5) using the Mouse PancChip 5.0 . Three assays were carried out for each time point.

2014-11-04 | E-MTAB-3071 | biostudies-arrayexpress

NR4A1 Inhibition Synergizes with Ibrutinib in Killing Mantle Cell Lymphoma Cells

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study are to identify NR4A1 targets by RNA-seq and high-throughput data analysis and verify these genes by quantitative reverse transcription polymerase chain reaction (qRT–PCR) methods. Methods: Jeko/Rec-1 Cas9 control and NR4A1 sgRNA stable cell lines were generated with tet-on system vector, sg RNAs were induced for 48 hours after doxycycline addition, mRNA was extracted and used for RNA sequencing. After TopHat analysis followed by Cufflinks, down/up regulated genes list was generated. qRT–PCR validation was then performed using SYBR Green assays. Results: Using an optimized data analysis workflow and with a fold change ≥1.3 and p value <0.05, thousands of genes were changed. Altered expression of 6 genes was then confirmed with qRT–PCR, demonstrating the high qulity of the RNA-seq method.

2017-10-25 | GSE106092 | GEO

RIP-seq for hnRNPK in Min6 cells

Project description:RNA immunoprecipitation followed by RNA-seq (RIP-seq) for hnRNPK was performed in Min6 cells treated with high glucose and palmitate levels for 30hrs. RNA-seq was performed on both immunoprecipitated RNA and total RNA for enrichment analysis.

2019-12-31 | GSE121296 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data