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Identification of ABA regulated genes in sunflower

ABSTRACT: Abiotic stress and more specifically drought is the major limiting factor for sunflower production. ABA is a key hormone for drought stress response in plants and sunflower. This experiment aims at identifying ABA responsive pathways in order to better understand sunflower responses to drought. We studied in parallel microRNA profiles on the same samples and we will try to identify sunflower microRNA regulated genes in response to ABA. The ultimate goal will be improve sunflower breeding through selection of key drought response genes.-The experiment consisted of 3 repeats of four 12-day-old-plantlets of sunflower genotype SF193 (INRA code: XRQ) grown in growth chamber conditions and submitted to a 6-hour-treatment of 10 µM absissic acid or not. Growth conditions were 14h light at 23°C and 10h night at 20°C under fluorescent bulbs. Plants were grown in 6 hydroponic boxes containing 20 litres of aerated liquid culture medium (as described in Massonneau et al., 2001 Planta). Leaves (not cotyledons) 1 to 4 were harvested 4 hours after light onset and frozen immediately in liquid nitrogen.

ORGANISM(S): Helianthus annuus

PROVIDER: GSE22519 | GEO | 2012/12/09

SECONDARY ACCESSION(S): PRJNA128483

REPOSITORIES: GEO

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Identification of ABA regulated genes in sunflower

Project description:Abiotic stress and more specifically drought is the major limiting factor for sunflower production. ABA is a key hormone for drought stress response in plants and sunflower. This experiment aims at identifying ABA responsive pathways in order to better understand sunflower responses to drought. We studied in parallel microRNA profiles on the same samples and we will try to identify sunflower microRNA regulated genes in response to ABA. The ultimate goal will be improve sunflower breeding through selection of key drought response genes.-The experiment consisted of 3 repeats of four 12-day-old-plantlets of sunflower genotype SF193 (INRA code: XRQ) grown in growth chamber conditions and submitted to a 6-hour-treatment of 10 ÂµM absissic acid or not. Growth conditions were 14h light at 23Â°C and 10h night at 20Â°C under fluorescent bulbs. Plants were grown in 6 hydroponic boxes containing 20 litres of aerated liquid culture medium (as described in Massonneau et al., 2001 Planta). Leaves (not cotyledons) 1 to 4 were harvested 4 hours after light onset and frozen immediately in liquid nitrogen. 6 arrays - SUNFLOWER; treated vs untreated comparison

2012-12-09 | E-GEOD-22519 | biostudies-arrayexpress

eqtl-Identification of expression QTL of ABA response in sunflower

Project description:affy_eqtl_sunflower - eqtl - Abiotic stress and more specifically drought is the major limiting factor for sunflower production. ABA is a key hormone for drought stress response in plants and sunflower. This experiment aims at identifying ABA responsive pathways in order to better understand sunflower responses to drought. The ultimate goal will be improve sunflower breeding through selection of key drought response genes. The experiment consisted of 1 or 2 (and 3 for the sample 209) repeats of 113 recombinant imbred lines and 11 repeats of the population parents SF1935 (INRA code XRQ), 11 SF326 (INRA code: PSC8) and the F1 hybrid 10 INEDI. The genotypes were grown in growth chamber conditions and submitted to a 6-hour-treatment of 0.5 µM absissic acid or not. Growth conditions were 14h light at 23°C and 10h night at 18°C under fluorescent bulbs. Plants were grown in 18 hydroponic boxes (9 for ABA treatment, 9 for control) containing 20 litres of aerated liquid culture medium (as described in Massonneau et al., 2001 Planta). Leaves (not cotyledons) from the 15-day-old-plantlets of sunflower were harvested 6 hours after treatment; the three repeats were pooled for each genotype and frozen immediately in liquid nitrogen.

2013-06-12 | GSE38812 | GEO

eqtl-Identification of expression QTL of ABA response in sunflower

Project description:affy_eqtl_sunflower - eqtl - Abiotic stress and more specifically drought is the major limiting factor for sunflower production. ABA is a key hormone for drought stress response in plants and sunflower. This experiment aims at identifying ABA responsive pathways in order to better understand sunflower responses to drought. The ultimate goal will be improve sunflower breeding through selection of key drought response genes. The experiment consisted of 1 or 2 (and 3 for the sample 209) repeats of 113 recombinant imbred lines and 11 repeats of the population parents SF1935 (INRA code XRQ), 11 SF326 (INRA code: PSC8) and the F1 hybrid 10 INEDI. The genotypes were grown in growth chamber conditions and submitted to a 6-hour-treatment of 0.5 ÂµM absissic acid or not. Growth conditions were 14h light at 23Â°C and 10h night at 18Â°C under fluorescent bulbs. Plants were grown in 18 hydroponic boxes (9 for ABA treatment, 9 for control) containing 20 litres of aerated liquid culture medium (as described in Massonneau et al., 2001 Planta). Leaves (not cotyledons) from the 15-day-old-plantlets of sunflower were harvested 6 hours after treatment; the three repeats were pooled for each genotype and frozen immediately in liquid nitrogen. 236 arrays - SUNFLOWER; treated vs untreated comparison Grp suffixes such as -A, -AA, and bis, are there to differentiate the same replicates.

2013-06-12 | E-GEOD-38812 | biostudies-arrayexpress

affy_strigolactone_sunflower-Identification of Strigolactone -regulated genes in sunflower

Project description:affy_strigolactone_sunflower - affy_strigolactone_sunflower - Abiotic stress and more specifically drought is the major limiting factor for sunflower production. Sunflower response to drought includes root plasticity to adapt to water availability and reach soil water. We identified genotype-specific responses of root architecture to strigolactone application. This experiment aims at identifying strigolactone responsive pathways in 6 genotypes in order to better understand molecular control of root development in sunflower and therefore its response to drought. The ultimate goal will be to improve sunflower breeding through selection of key drought response genes.-The experiment consisted of 2 repeats of four 12-day-old-plantlets of 6 sunflower genotype SF193 (INRA code: XRQ), SF326 (INRA code: PSC8), SF056 (INRA code: FU), SF306 (INRA code: PAZ2), SF302 (INRA code: PAC2), SF268 (INRA code: RHA266), grown in growth chamber conditions and submitted to a 24-hour-treatment of 100 nM strigolactone analogue rac-GR24 (Chiralix, Nijmegen, Netherland) or not. Growth conditions were 14h light at 23°C and 10h night at 20°C under fluorescent bulbs. Plants were grown in hydroponic boxes containing 20 litres of aerated liquid culture medium (as described in Massonneau et al., 2001 Planta). The entire root systems were harvested 4 hours after light onset and frozen immediately in liquid nitrogen.

2013-05-06 | GSE25888 | GEO

affy_strigolactone_sunflower-Identification of Strigolactone -regulated genes in sunflower

Project description:affy_strigolactone_sunflower - affy_strigolactone_sunflower - Abiotic stress and more specifically drought is the major limiting factor for sunflower production. Sunflower response to drought includes root plasticity to adapt to water availability and reach soil water. We identified genotype-specific responses of root architecture to strigolactone application. This experiment aims at identifying strigolactone responsive pathways in 6 genotypes in order to better understand molecular control of root development in sunflower and therefore its response to drought. The ultimate goal will be to improve sunflower breeding through selection of key drought response genes.-The experiment consisted of 2 repeats of four 12-day-old-plantlets of 6 sunflower genotype SF193 (INRA code: XRQ), SF326 (INRA code: PSC8), SF056 (INRA code: FU), SF306 (INRA code: PAZ2), SF302 (INRA code: PAC2), SF268 (INRA code: RHA266), grown in growth chamber conditions and submitted to a 24-hour-treatment of 100 nM strigolactone analogue rac-GR24 (Chiralix, Nijmegen, Netherland) or not. Growth conditions were 14h light at 23Â°C and 10h night at 20Â°C under fluorescent bulbs. Plants were grown in hydroponic boxes containing 20 litres of aerated liquid culture medium (as described in Massonneau et al., 2001 Planta). The entire root systems were harvested 4 hours after light onset and frozen immediately in liquid nitrogen. 24 arrays - SUNFLOWER; treated vs untreated comparison

2013-05-06 | E-GEOD-25888 | biostudies-arrayexpress

affy_sunflower_2010_13-ROS and hormones in sunflower seed dormancy.

Project description:affy_sunflower_2010_13 - affy_sunflower_2010_13 - It concerns the interaction between ROS and hormones in dormancy release in sunflower seeds. ABA is responsible for dormancy maintenance, while GA and ethylene promote seed germination. Based on our results, ROS could represent good candidate to shift from a hormone signalling to another determining the dormancy state in sunflower seeds.-We aim to understand the mechanisms controlling sunflower seed dormancy at the transcriptomic level, by the application of treatments which maintain dormancy as ABA, or alleviate dormancy as ROS and ethylene. Transcripts comparison will be performed between dormant and non-dormant sunflower embryo imbibed 24h on water, on ABA, on methylviologen, a pro-oxidant compound or on ethylene.

2013-08-01 | GSE44165 | GEO

affy_sunflower_2010_13-ROS and hormones in sunflower seed dormancy.

2013-08-01 | E-GEOD-44165 | biostudies-arrayexpress

affy_sunflower_2011_02-Effect of frost on sunflower

Project description:affy_sunflower_2011_02 - affy_sunflower_2011_02 - The early sowing constitutes an alternative strategy to avoid drought occurring during flowering and post-flowering periods and responsible for decrease in sunflower production. In French cropping system, early sowing is associated to low temperature period and frost during first development stages in sunflower. Knowledge about metabolism of frost acclimation must be performed to supply tools for breeding programs in sunflower. The aim of our experiment is to unravel the transcriptional regulation underpinning frost tolerance in sunflower-5 genotypes of sunflower were grown in a growth chamber 1 (23°C day/18°C night, 63% air humidity, 14 hours day photoperiod). At the stage of 6 leaves well-developed, 12 plants of each genotype were subjected to cold acclimation (+4°C during 2 days) in another growth chamber 2. Then, these plants were subjected to 2 nights at -3°C (frost treatment). Chlorophylle fluorescence, Osmotic potential, Relative electrolyte leakage were then determined in the following conditions : - 6th October on 6 plants X 5 genotypes from growth chamber 1 (C1) - 6th October on 6 plants X 5 genotypes from growth chamber 2 (S1) - 11th October on 6 plants X 5 genotypes from growth chamber 1 (C2) - 11th October on 6 plants X 5 genotypes from growth chamber 1 (S2)

2013-09-01 | GSE45586 | GEO

Transcriptomic data of leaves from eight sunflower lines and their sixteen hybrids under water deficit

Project description:Twenty-four sunflower genotypes were selected to represent genetic diversity within cultivated sunflower and included both inbred lines and their hybrids. Drought stress was applied to plants in pots at the vegetative stage using the high-throughput phenotyping platform Heliaphen. Here, we provide transcriptomics data from sunflower leaves. These data differentiate both treatment and the different genotypes and constitute a valuable resource to the community to study adaptation of crops to drought and the transcriptomic basis of heterosis.

2020-09-22 | GSE145709 | GEO

affy_sunflower_2011_02-Effect of frost on sunflower

Project description:affy_sunflower_2011_02 - affy_sunflower_2011_02 - The early sowing constitutes an alternative strategy to avoid drought occurring during flowering and post-flowering periods and responsible for decrease in sunflower production. In French cropping system, early sowing is associated to low temperature period and frost during first development stages in sunflower. Knowledge about metabolism of frost acclimation must be performed to supply tools for breeding programs in sunflower. The aim of our experiment is to unravel the transcriptional regulation underpinning frost tolerance in sunflower-5 genotypes of sunflower were grown in a growth chamber 1 (23Â°C day/18Â°C night, 63% air humidity, 14 hours day photoperiod). At the stage of 6 leaves well-developed, 12 plants of each genotype were subjected to cold acclimation (+4Â°C during 2 days) in another growth chamber 2. Then, these plants were subjected to 2 nights at -3Â°C (frost treatment). Chlorophylle fluorescence, Osmotic potential, Relative electrolyte leakage were then determined in the following conditions : - 6th October on 6 plants X 5 genotypes from growth chamber 1 (C1) - 6th October on 6 plants X 5 genotypes from growth chamber 2 (S1) - 11th October on 6 plants X 5 genotypes from growth chamber 1 (C2) - 11th October on 6 plants X 5 genotypes from growth chamber 1 (S2) 12 arrays - SUNFLOWER; treated vs untreated comparison

2013-09-01 | E-GEOD-45586 | biostudies-arrayexpress

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