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Chromatin opening ability of pioneer factor Pax7 depends on unique isoform and C-terminal domain

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE225231 | GEO | 2023/06/17

REPOSITORIES: GEO

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Chromatin opening ability of pioneer factor Pax7 depends on unique isoform and C-terminal domain (RNA-Seq)

Project description:Pioneer factors are transcription factors (TFs) that have the unique ability to recognise their target DNA sequences within closed chromatin. Whereas their interactions with cognate DNA is similar to other TFs, their ability to interact with chromatin remains poorly understood. Having previously defined the modalities of DNA interactions for the pioneer factor Pax7, we have now used natural isoforms of this pioneer as well as deletion and replacement mutants to investigate the Pax7 structural requirements for chromatin interaction and opening. We show that the GL+ natural isoform of Pax7 that has two extra amino acids within the DNA binding paired domain is unable to activate the melanotrope transcriptome and to fully activate a large subset of melanotrope-specific enhancers targeted for Pax7 pioneer action. This enhancer subset remains in the primed state rather than being fully activated, despite the GL+ isoform having similar intrinsic transcriptional activity as the GL- isoform. C-terminal deletions of Pax7 lead to the same loss of pioneer ability, with similar reduced recruitments of the cooperating TF Tpit and of the co-regulators Ash2 and BRG1. This suggest complex interrelations between the DNA binding and C-terminal domains of Pax7 that are crucial for its chromatin opening pioneer ability.

2023-06-20 | GSE225229 | GEO

Chromatin opening ability of pioneer factor Pax7 depends on unique isoform and C-terminal domain (ChIP-Seq)

2023-06-17 | GSE225228 | GEO

Chromatin opening ability of pioneer factor Pax7 depends on unique isoform and C-terminal domain (ATAC-Seq)

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Chromatin opening ability of pioneer factor Pax7 depends on unique isoform and C-terminal domain

Project description:Chromatin opening ability of pioneer factor Pax7 depends on unique isoform and C-terminal domain

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Chromatin opening ability of pioneer factor Pax7 depends on unique isoform and C-terminal domain (RNA-Seq)

Project description:Chromatin opening ability of pioneer factor Pax7 depends on unique isoform and C-terminal domain (RNA-Seq)

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Chromatin opening ability of pioneer factor Pax7 depends on unique isoform and C-terminal domain (ChIP-Seq)

Project description:Chromatin opening ability of pioneer factor Pax7 depends on unique isoform and C-terminal domain (ChIP-Seq)

| PRJNA934462 | ENA

Chromatin opening ability of pioneer factor Pax7 depends on unique isoform and C-terminal domain (ATAC-Seq)

Project description:Chromatin opening ability of pioneer factor Pax7 depends on unique isoform and C-terminal domain (ATAC-Seq)

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Pioneer factor Pax7 initiates two-step cell-cycle dependent chromatin opening

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-10-08 | GSE240090 | GEO

Pioneer factor Pax7 initiates two-step cell-cycle dependent chromatin opening (ATAC-seq)

Project description:Pioneer transcription factors direct cell differentiation by deploying new enhancer repertoires through their unique ability to target and initiate remodelling of closed chromatin. The initial steps of their action remain undefined although pioneers were shown to interact with nucleosomal target DNA and with some chromatin remodelling complexes. We now define the sequence of events that enable the pioneer Pax7 with its unique abilities. Chromatin condensation exerted by linker histone H1 is the first constraint on Pax7 recruitment, and this establishes the initial speed of chromatin remodelling. The first step of pioneer action involves recruitment of the KDM1A (Lsd1) H3K9me2 demethylase for removal of this repressive mark, as well as recruitment of the MLL complex for deposition of the activating H3K4me1 mark. Further progression of pioneer action requires passage through cell division, and this involves dissociation of pioneer targets from perinuclear lamin B. Only then, the SWI/SNF remodeling complex and the coactivator p300 are recruited, leading to nucleosome displacement and enhancer activation. Thus, the unique features of pioneer actions are those occurring in the lamin-associated compartment of the nucleus. This model is consistent with prior work that showed a dependence on cell division for establishment of new cell fates.

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Pioneer factor Pax7 initiates two-step cell-cycle dependent chromatin opening (ChIP-seq)

2023-10-08 | GSE212390 | GEO