Efficient and sensitive profiling of RNA-protein interactions using TLC-CLIP [dataset 2]

ABSTRACT: RNA-binding proteins are instrumental for post-transcriptional gene regulation, yet transcriptome-wide methods to profile RNA-protein interactions remain technically challenging. We present an improved library preparation strategy for cross-linking and immunoprecipitation (CLIP) that involves tailing and ligation of cDNA molecules (TLC) for increased sensitivity and efficiency. TLC-CLIP eliminates time-consuming purifications, reduces sample loss, and minimises experimental steps, allowing precise profiling of RNA-protein interactions from limited starting material at nucleotide resolution.

ORGANISM(S): Homo sapiens

PROVIDER: GSE225357 | GEO | 2023/05/10

REPOSITORIES: GEO