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Effects of the conditioned medium derived from human adipose-derived mesenchymal stem cells (hAdMSC-CM) on Benzalkonium Chloride (BAC)-treated human corneal epithelial cells.

ABSTRACT: To investigate the mechanism underlying effects of hAdMSC-CM on BAC-induced cytotoxicity, inflammation, and barrier dysfunction in human corneal epithelial cells. We confirmed that the the effects of hAdMSC-CM were associated with the TGFβ and JAK-STAT signalling pathways.

ORGANISM(S): Homo sapiens

PROVIDER: GSE225408 | GEO | 2024/09/30

REPOSITORIES: GEO

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Benzalkonium chloride accelerates the formation of the amyloid fibrils of corneal dystrophy-associated peptides.

Project description:Corneal dystrophies are genetic disorders resulting in progressive corneal clouding due to the deposition of amyloid fibrils derived from keratoepithelin, also called transforming growth factor ?-induced protein (TGFBI). The formation of amyloid fibrils is often accelerated by surfactants such as sodium dodecyl sulfate (SDS). Most eye drops contain benzalkonium chloride (BAC), a cationic surfactant, as a preservative substance. In the present study, we aimed to reveal the role of BAC in the amyloid fibrillation of keratoepithelin-derived peptides in vitro. We used three types of 22-residue synthetic peptides covering Leu110-Glu131 of the keratoepithelin sequence: an R-type peptide with wild-type R124, a C-type peptide with C124 associated with lattice corneal dystrophy type I, and a H-type peptide with H124 associated with granular corneal dystrophy type II. The time courses of spontaneous amyloid fibrillation and seed-dependent fibril elongation were monitored in the presence of various concentrations of BAC or SDS using thioflavin T fluorescence. BAC and SDS accelerated the fibrillation of all synthetic peptides in the absence and presence of seeds. Optimal acceleration occurred near the CMC, which suggests that the unstable and dynamic interactions of keratoepithelin peptides with amphipathic surfactants led to the formation of fibrils. These results suggest that eye drops containing BAC may deteriorate corneal dystrophies and that those without BAC are preferred especially for patients with corneal dystrophies.

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Gene expression profile of human dental follicle cells (hDFCs) induced by conditioned medium derived from human dental pulp cells (hDPCs-CM)

Project description:The purpose of this study was to examine the effects of hDPCs-CM on osteogenic differentiation of hDFCs and to compare gene expression in hDFCs in the presence or absence of hDPCs-CM

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Extensive modulation of inflammatory and axonal genes led to robust axon regeneration in severe benzalkonium chloride-injured corneas treated with cord blood-derived biologics

Project description:Modern ophthalmic solutions contain benzalkonium chloride (BAK), a potent antimicrobial preservative, that chronically damages the ocular apparatus. Umbilical cord blood serum (UCS) and platelet-rich plasma (UCPRP) are promising treatments for BAK-injured corneas. Here, for the first time, the feasibility of using both of these biologics for corneal treatment was demonstrated. Using animal studies and RNA-seq analysis, it was validated that severe BAK injury led to the upregulation of innate and adaptive immune responses, which corresponded to morphological and pathological changes in the cornea. When UCS and UCPRP were administered, besides significant corneal recovery, widespread immune pathways downregulation was observed. Subsequent verification of some of the genes involved in two of the most notable KEGG pathways suggested that the amelioration of inflammation was likely due to the reduction of immune cells, the decrease in chemoattractants, as well as the lessening of pro-inflammatory MMPs. Besides that, regulation of several key axonal genes led to robust axon recovery that correlated to nearly restored corneal sensitivity. Thus, these findings demonstrated the viability of cord blood-derived therapeutics for treating sustained, BAK-induced injuries in the cornea and also provided evidence that an optimal level of inflammatory response is crucial for stimulating axon regeneration.

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Expression data from human iPSC-derived corneal epithelial cells

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Aspergilli Response to Benzalkonium Chloride and Novel-Synthesized Fullerenol/Benzalkonium Chloride Nanocomposite.

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Gene expression analyses of HUVECs treated with conditioned medium derived from bone marrow mesenchymal stromal cells (BMSC-CM)

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Human embryonic stem cell-derived corneal endothelial cells

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2017-02-09 | GSE70954 | GEO

proteins identified in biological activated carbon (BAC) filter

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