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ScRNA-seq of brain cells from control and 3xDR mouse brains

ABSTRACT: As important immune cells, microglia undergo a series of alterations during aging that increase the susceptibility to brain dysfunctions. However, the longitudinal characteristics of microglia remain elusive. In this study, we mapped the transcriptional and epigenetic profiles of microglia from 3- to 24-month-old mice. We observed unexpected sex differences and identified age-dependent microglia (ADEM) genes during the aging process. We then compared the characteristics of aging and reactivity in female microglia at the single-cell resolution and epigenetic level. To dissect the functions of aged microglia excluding the influence from other aged brain cells, we established an accelerated microglial turnover model without directly affecting other brain cells. By this model, we achieved aged microglia in non-aged brains and confirmed that aged microglia per se contribute to cognitive decline. Collectively, our work provides a comprehensive resource for decoding the aging process of microglia, shedding light on how microglia maintain brain functions.

ORGANISM(S): Mus musculus

PROVIDER: GSE226286 | GEO | 2023/07/25

REPOSITORIES: GEO

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