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5-formylcytosine is an activating epigenetic mark for RNA Pol III during zygotic reprogramming

ABSTRACT: 5-methylcytosine (5mC) is an established epigenetic mark in vertebrate genomic DNA, but whether its oxidation intermediates formed during TET-mediated DNA demethylation possess an instructive role of their own that is also physiologically relevant remains unresolved. Here, we reveal a 5-formylcytosine (5fC) nuclear chromocenter, which transiently forms during zygotic genome activation (ZGA) in Xenopus and mouse embryos. We identify this chromocenter as the perinucleolar compartment, a structure associated with RNA Pol III transcription. In Xenopus embryos, 5fC is highly enriched on Pol III target genes activated at ZGA, notably at oocyte-type tandem arrayed tRNA genes. By manipulating Tet and Tdg enzymes, we show that 5fC is required as a regulatory mark to promote Pol III recruitment as well as tRNA expression. Concordantly, 5fC modification of a tRNA transgene enhances its expression in vivo. The results establish 5fC as an activating epigenetic mark during zygotic reprogramming of Pol III gene expression.

ORGANISM(S): Xenopus tropicalis

PROVIDER: GSE226467 | GEO | 2024/08/29

REPOSITORIES: GEO

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5-formylcytosine is an activating epigenetic mark for RNA Pol III during zygotic reprogramming

Project description:5-formylcytosine is an activating epigenetic mark for RNA Pol III during zygotic reprogramming

| PRJNA940392 | ENA

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